Ahmad Bashir, Shireen Farah, Bashir Shumaila, Khan Ibrar, Azam Sadiq
Center of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan.
Department of Pharmacy, University of Peshawar, Pakistan.
IET Nanobiotechnol. 2016 Oct;10(5):281-287. doi: 10.1049/iet-nbt.2015.0053.
The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) of , and leaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano-particles was confirmed by transmission electron microscopy (30-150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds. AgNPs showed significant (96%) activity against , (95%) and (89%). Good antioxidant activity was shown by AgNPs at 300 µl (79%). AgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.
本研究旨在合成来自[植物名称1]、[植物名称2]和[植物名称3]叶子的稳定、环保且生物相容的银纳米颗粒（AgNPs），并对其生物活性进行筛选。紫外可见光谱分析表明，AgNPs的λ-最大值范围为350 - 500 nm。在X射线衍射测量中，所有AgNPs均具有多晶结构，表现为20个值（范围为10 - 80°）的完整光谱中的强烈图形峰，并得到扫描电子显微镜数据的支持。通过透射电子显微镜确认纳米颗粒的尺寸为30 - 150 nm。通过同步热重分析和差示热分析评估了不同温度下的质量损失，结果表明随着温度从200升高到800°C，化合物的质量和活性降低，因此得出其为热敏感化合物的结论。AgNPs对[细菌名称1]表现出显著（96%）的活性，对[细菌名称2]为（95%），对[细菌名称3]为（89%）。[植物名称1] AgNPs在300 μl时表现出良好的抗氧化活性（79%）。[植物名称2] AgNPs在最高浓度下表现出显著的植物毒性活性（88%）。测试样品未观察到血凝反应。上述结果表明，从所选植物物种合成的AgNPs具有显著的抗菌和植物毒性作用。
