Abdul Qadir Muhammad, Shahzadi Syeda Kiran, Bashir Asad, Munir Adil, Shahzad Shabnam
Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
Department of Chemistry, Minhaj University, Lahore 54590, Pakistan.
Int J Anal Chem. 2017;2017:3475738. doi: 10.1155/2017/3475738. Epub 2017 Feb 16.
The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (), garlic (), mint (), thyme (), oak (), aloe vera ( Miller), and ginger (). All extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Different extracts inhibited oxidation of linoleic acid by 16.6-84.2% while DPPH radical scavenging activity (IC values) ranged from 17.8% to 79.1 g/mL. Reducing power at 10 mg/mL extract concentration ranged from 0.11 to 0.84 nm. Furthermore the extracts of these medicinal herbs in 80% methanol, 80% ethanol, 80% acetone, and 100% water were screened for antimicrobial activity by disc diffusion method against selected bacterial strains, , , , and , and fungal strains, , , and . The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains. Results of various assays were analyzed statistically by applying appropriate statistical methods.
本研究旨在评估洋葱（）、大蒜（）、薄荷（）、百里香（）、橡木（）、芦荟（米勒）和生姜（）的酚类、黄酮类含量以及抗氧化和抗菌活性。所有提取物的总酚含量范围很广，即4.96至98.37毫克/100克没食子酸当量，总黄酮含量范围为0.41至17.64毫克/100克儿茶素当量。通过测量还原能力、使用亚油酸系统抑制过氧化作用以及2,2-二苯基-1-苦基肼自由基（DPPH）清除活性来测定抗氧化活性（AA）。不同提取物对亚油酸氧化的抑制率为16.6 - 84.2%，而DPPH自由基清除活性（IC值）范围为17.8%至79.1微克/毫升。提取物浓度为10毫克/毫升时的还原能力范围为0.11至0.84纳米。此外，采用纸片扩散法对这些草药在80%甲醇、80%乙醇、80%丙酮和100%水中的提取物针对选定的细菌菌株、、、和以及真菌菌株、、和进行抗菌活性筛选。与真菌菌株相比，提取物对细菌菌株显示出更好的抗菌活性。通过应用适当的统计方法对各种测定结果进行统计学分析。
