Beltrán Roberto, Valls Aina, Cebrián Nuria, Zornoza Carlos, García Breijo Francisco, Reig Armiñana José, Garmendia Alfonso, Merle Hugo
Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València, Valencia, Spain.
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia, Valencia, Spain.
PeerJ. 2019 Dec 11;7:e8195. doi: 10.7717/peerj.8195. eCollection 2019.
Between February 2018 and April 2018, flowers were collected from eight Rosaceae species. Flowers were kept in a freezer at -20 °C for three freezing times (Treatment 1, two months; Treatment 2, four months; Treatment 3, six months). After extracting pollen, germination was induced in a culture medium and incubated at six different temperatures for 72 h. The percentage of pollen germination, average pollen tube length and maximum pollen tube length were measured. Pollen germination was maximum for all species between 15 °C and 30 °C. , and obtained 30-52% pollen germination between 15 °C and 20 °C. had 40% pollen germination at 30 °C. All species studied reached the maximum pollen tube length between 10 °C and 25 °C. Germination did not change significantly for any of the species with freezing time, but we found significant differences in the three parameters measured between treatments. The highest germination percentages were obtained in Treatment 2 (four months frozen at -20 °C), while the maximum pollen tube length was reached in Treatment 1 (two months frozen at -20 °C). According to our results, freezing time affected the germination-temperature patterns. This could indicate that studies on the effect of temperature on pollen germination should always be carried out with fresh pollen to obtain more conclusive data.
2018年2月至2018年4月期间，采集了8种蔷薇科植物的花朵。将花朵置于-20°C的冰箱中冷冻三次（处理1，两个月；处理2，四个月；处理3，六个月）。提取花粉后，在培养基中诱导萌发，并在6个不同温度下孵育72小时。测量花粉萌发率、花粉管平均长度和花粉管最大长度。所有物种在15°C至30°C之间花粉萌发率最高。 ， 在15°C至20°C之间花粉萌发率为30%-52%。 在30°C时花粉萌发率为40%。所有研究的物种在10°C至25°C之间达到花粉管最大长度。对于任何物种，萌发率均未随冷冻时间而显著变化，但我们发现各处理间所测的三个参数存在显著差异。处理2（在-20°C冷冻四个月）获得的萌发率最高，而处理1（在-20°C冷冻两个月）达到花粉管最大长度。根据我们的结果，冷冻时间影响萌发-温度模式。这可能表明，关于温度对花粉萌发影响的研究应始终使用新鲜花粉进行，以获得更具结论性的数据。
