Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València, Valencia, Spain.
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia, Valencia, Spain.
PeerJ. 2023 Feb 7;11:e14775. doi: 10.7717/peerj.14775. eCollection 2023.
This study aims to elucidate whether sulfur can inhibit citrus pollination by affecting pollen grains. For this, four sulfur-based products (inorganic sulfur, water dispersible granular sulfur, ammonium sulfate, copper sulfate) were tested to evaluate their effect on pollen germination and pollen tube growth of two citrus varieties: Clemenules mandarin () and Nova tangelo ( x [ x ]). Pollen grains were extracted from the flowers of these two varieties and subsequently placed in Petri dishes with modified BK (boron and potassium) germination medium with six concentrations of the sulfur-based products (0.2, 2, 20, 200, 2,000, 20,000 mg l). All the dishes were incubated and the pollen germination rate was calculated. All the sulfur products showed progressive pollen germination inhibition with a rising sulfur concentration. CTC50 (50% cytotoxicity inhibition) was around 20 mg l, with significant differences among treatments. Total pollen germination inhibition took place at 20,000 mg l. These results demonstrate that sulfur application can affect citrus pollination.
本研究旨在阐明硫是否可以通过影响花粉粒来抑制柑橘授粉。为此，测试了四种含硫产品（无机硫、水分散粒状硫磺、硫酸铵、硫酸铜），以评估它们对两种柑橘品种（克莱门氏柠檬（）和 Nova 橘柚（））花粉萌发和花粉管生长的影响。从这两个品种的花朵中提取花粉粒，然后将其放置在含有改良 BK（硼和钾）萌发培养基的培养皿中，培养基中含有六种浓度的含硫产品（0.2、2、20、200、2000、20000mg/L）。所有培养皿均进行孵育，并计算花粉萌发率。所有含硫产品均表现出随着硫浓度的升高而逐渐抑制花粉萌发。CTC50（50%细胞毒性抑制）约为 20mg/L，各处理之间存在显著差异。在 20000mg/L 时，花粉完全被抑制萌发。这些结果表明，硫的应用会影响柑橘授粉。
