平古病毒：来自南极洲企鹅体内的一种新型微小核糖核酸病毒。
Pingu virus: A new picornavirus in penguins from Antarctica.
作者信息
de Souza William Marciel, Fumagalli Marcílio Jorge, Martin Matheus Cavalheiro, de Araujo Jansen, Orsi Maria Angela, Sanfilippo Luiz Francisco, Modha Sejal, Durigon Edison Luiz, Proença-Módena José Luiz, Arns Clarice Weis, Murcia Pablo Ramiro, Figueiredo Luiz Tadeu Moraes
机构信息
Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Avenida dos Bandeirantes 3900, Ribeirão Preto, SP, 14049-900 Brazil.
Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, 255Cidade Universitária "Zeferino Vaz", Campinas, SP, 13083-862 Brazil.
出版信息
Virus Evol. 2019 Dec 13;5(2):vez047. doi: 10.1093/ve/vez047. eCollection 2019 Jul.
family comprises single-stranded, positive-sense RNA viruses distributed into forty-seven genera. Picornaviruses have a broad host range and geographic distribution in all continents. In this study, we applied a high-throughput sequencing approach to examine the presence of picornaviruses in penguins from King George Island, Antarctica. We discovered and characterized a novel picornavirus from cloacal swab samples of gentoo penguins (), which we tentatively named Pingu virus. Also, using RT-PCR we detected this virus in 12.9 per cent of cloacal swabs derived from , but not in samples from adélie penguins () or chinstrap penguins (). Attempts to isolate the virus in a chicken cell line and in embryonated chicken eggs were unsuccessful. Our results expand the viral diversity, host range, and geographical distribution of the .
该病毒科包含单链正义RNA病毒，分为47个属。小RNA病毒具有广泛的宿主范围，在各大洲均有地理分布。在本研究中，我们应用高通量测序方法检测了南极乔治王岛企鹅体内小RNA病毒的存在情况。我们从巴布亚企鹅的泄殖腔拭子样本中发现并鉴定了一种新型小RNA病毒，我们暂时将其命名为平古病毒。此外，我们通过逆转录聚合酶链反应在12.9%的巴布亚企鹅泄殖腔拭子中检测到了这种病毒，但在阿德利企鹅或帽带企鹅的样本中未检测到。尝试在鸡细胞系和鸡胚中分离该病毒均未成功。我们的结果扩展了小RNA病毒的病毒多样性、宿主范围和地理分布。
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