College of Chemistry and Materials Science, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Wenyuan Road, Nanjing, Jiangsu 210023, P. R. China.
J Mater Chem B. 2020 Jan 22;8(3):534-545. doi: 10.1039/c8tb02430h.
Photodynamic therapy (PDT) is strongly O2 dependent. Therefore, its therapeutic effects are seriously hindered in hypoxic tumors. Red blood cells are responsible for delivering O2 in the blood. In this manuscript, biomimetic red blood cells (BRBCs) were exploited using a layer-by-layer assembly method, using Fe3O4@CuO, oxyhemoglobin (OxyHb), a photosensitizer and a photo-cross linked acrylate modified hyaluronic acid (HA) gel shell. The Fe3O4@CuO core has very high OxyHb loading efficiency (the adsorption capacity of Fe3O4@CuO for OxyHb is derived to be 0.99 mg mg-1) to ensure a sufficient O2 supply. OxyHb was protected well by the HA shell in order to avoid O2 release during the delivery process in blood before arrival at the tumor tissue. The HA shell protection can be eliminated in position at the tumor to trigger O2 release through hyaluronidase (HAase) triggered HA degradation. Furthermore, Fe3O4 in the nanosystem can provide magnetic field assisted tumor targeting and magnetic resonance imaging of the tumor. Therefore, this work presents a highly efficient all-in-one biomimetic nanomedicine approach to overcome hypoxia and achieve tumor targeting theranostics.
光动力疗法（PDT）强烈依赖于 O2。因此，其治疗效果在缺氧肿瘤中受到严重阻碍。红细胞负责在血液中输送 O2。在本文中，使用层层组装方法利用仿生红细胞（BRBC），使用 Fe3O4@CuO、氧合血红蛋白（OxyHb）、光敏剂和光交联丙烯酰胺修饰的透明质酸（HA）凝胶壳。Fe3O4@CuO 核具有非常高的 OxyHb 负载效率（Fe3O4@CuO 对 OxyHb 的吸附能力被推导为 0.99 mg mg-1），以确保有足够的 O2 供应。OxyHb 被 HA 壳很好地保护，以避免在到达肿瘤组织之前在血液输送过程中释放 O2。HA 壳的保护可以在肿瘤部位消除，通过透明质酸酶（HAase）触发透明质酸降解来触发 O2 释放。此外，纳米系统中的 Fe3O4 可以提供磁场辅助肿瘤靶向和肿瘤磁共振成像。因此，这项工作提出了一种高效的一体化仿生纳米医学方法，以克服缺氧并实现肿瘤靶向治疗。
