碳水化合物对刀豆球蛋白A和植物血凝素所致豹蛙（北美豹蛙）红细胞凝集活性的抑制特异性

Specificity of carbohydrate inhibition on leopard frog (Rana pipiens) erythrocyte agglutinating activity by concanavalin A and phytohemagglutinin.

Wright R K, Cooper E L

Department of Anatomy, School of Medicine, University of California, Los Angeles 90024.

Comp Biochem Physiol B. 1979;63(1):13-8.

Abstract

Leopard frog (Rana pipiens) erythrocytes are agglutinated by the plant lectins concanavalin A and phytohemagglutinin. 2. Concanavalin A hemagglutination can be specifically inhibited by the sugars mannose, glucose, arabohexulose, alpha-methyl-D-mannopyranoside, N-acetyl-D-glucosamine, maltose, sucrose, trehalose and melezitose. 3. Sugars inhibiting concanavalin A hemagglutination are structurally composed of alpha-D-glucopyranosyl, alpha-D-mannopyranosyl or beta-D-fructofuranosyl residues with the same hydroxyl groups at carbons 3, 4 and 6. 4. Phytohemagglutinin hemagglutination can only be inhibited by the sugar N-acetyl-D-galactosamine. 5. Sugar inhibition of concanavalin A and phytohemagglutinin leopard frog erythrocyte agglutination is the same as that observed in many mammalian species.

摘要

豹蛙（北美林蛙）红细胞可被植物凝集素刀豆球蛋白A和植物血凝素凝集。2. 刀豆球蛋白A的血细胞凝集作用可被甘露糖、葡萄糖、阿洛己糖、α-甲基-D-甘露吡喃糖苷、N-乙酰-D-葡糖胺、麦芽糖、蔗糖、海藻糖和松三糖特异性抑制。3. 抑制刀豆球蛋白A血细胞凝集作用的糖类在结构上由α-D-吡喃葡糖基、α-D-吡喃甘露糖基或β-D-呋喃果糖基残基组成，在碳3、4和6处具有相同的羟基。4. 植物血凝素的血细胞凝集作用只能被糖类N-乙酰-D-半乳糖胺抑制。5. 糖类对刀豆球蛋白A和植物血凝素豹蛙红细胞凝集作用的抑制与在许多哺乳动物物种中观察到的情况相同。