CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR 3572 , University of Strasbourg , 67000 Strasbourg , France.
Department of Life Sciences , University of Trieste , 34127 Trieste , Italy.
ACS Chem Neurosci. 2020 Jan 15;11(2):162-172. doi: 10.1021/acschemneuro.9b00522. Epub 2020 Jan 3.
Peptides constituted of backbone homologated α-amino acids combined with carbon materials offer interesting possibilities in the modulation of cellular functions. In this work, we have prepared diphenylalanine β- and γ-peptides and conjugated them to carbon nanotubes (CNTs). These hybrids were able to self-assemble into fibrillar dendritic structures enabling the growth of primary hippocampal cells and the modulation of their neuronal functions. In particular, following the deposition of the different nanomaterials on glass substrates, we have evaluated their effects on circuit function and geometry. The geometrical restrictions due to CNT nucleated nodes allowed growth of neuronal networks with control over network geometry, and exploring its functional impact. In diverse applications from basic neuroscience, the presence of CNT nodes may be exploited in brain interfaces able to convey highly localized electrical stimuli.
由骨架同源α-氨基酸与碳材料结合而成的肽在调节细胞功能方面提供了有趣的可能性。在这项工作中，我们制备了二苯丙氨酸β-和γ-肽，并将它们与碳纳米管（CNT）结合。这些杂化物能够自组装成纤维状树枝状结构，从而促进原代海马细胞的生长，并调节其神经元功能。特别是，在将不同的纳米材料沉积在玻璃基底上之后，我们评估了它们对电路功能和几何形状的影响。由于 CNT 引发的节点引起的几何限制允许具有控制网络几何形状的神经元网络生长，并探索其功能影响。在从基础神经科学到各种应用中，CNT 节点的存在可用于能够传递高度局部化电刺激的脑接口。
