Guilbaud-Chéreau Chloé, Dinesh Bhimareddy, Wagner Laurène, Chaloin Olivier, Ménard-Moyon Cécilia, Bianco Alberto
CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR 3572, University of Strasbourg, ISIS, 67000 Strasbourg, France.
Nanomaterials (Basel). 2022 May 11;12(10):1643. doi: 10.3390/nano12101643.
Peptide-based hydrogels are considered of special importance due to their biocompatibility and biodegradability. They have a wide range of applications in the biomedical field, such as drug delivery, tissue engineering, wound healing, cell culture media, and biosensing. Nevertheless, peptide-based hydrogels composed of natural α-amino acids are limited for in vivo applications because of the possible degradation by proteolytic enzymes. To circumvent this issue, the incorporation of extra methylene groups within the peptide sequence and the protection of the terminal amino group can increase the enzymatic stability. In this context, we investigated the self-assembly capacity of aromatic dipeptides (Boc-α-diphenylalanine and Boc-α-dityrosine) and their β- and γ-homologues and developed stable hydrogels. Surprisingly, only the Boc-diphenylalanine analogues were able to self-assemble and form hydrogels. A model drug, l-ascorbic acid, and oxidized carbon nanotubes (CNTs) or graphene oxide were then incorporated into the hydrogels. Under near-infrared light irradiation, the photothermal effect of the carbon nanomaterials induced the destabilization of the gel structure, which caused the release of a high amount of drug, thus providing opportunities for photocontrolled on-demand drug release.
基于肽的水凝胶因其生物相容性和可生物降解性而被认为具有特殊重要性。它们在生物医学领域有广泛应用，如药物递送、组织工程、伤口愈合、细胞培养基和生物传感。然而，由天然α-氨基酸组成的基于肽的水凝胶在体内应用中受到限制，因为可能会被蛋白水解酶降解。为了规避这个问题，在肽序列中引入额外的亚甲基以及保护末端氨基可以提高酶稳定性。在此背景下，我们研究了芳香二肽（Boc-α-二苯基丙氨酸和Boc-α-二酪氨酸）及其β-和γ-同系物的自组装能力，并开发了稳定的水凝胶。令人惊讶的是，只有Boc-二苯基丙氨酸类似物能够自组装并形成水凝胶。然后将模型药物l-抗坏血酸以及氧化碳纳米管（CNTs）或氧化石墨烯掺入水凝胶中。在近红外光照射下，碳纳米材料的光热效应导致凝胶结构不稳定，从而释放大量药物，为光控按需药物释放提供了机会。
