mA RNA modification modulates gene expression and cancer-related pathways in clear cell renal cell carcinoma.

To systematically profile the global mA modification pattern in clear cell renal cell carcinoma (ccRCC). mA modification patterns in ccRCC and normal tissues were described via mA sequencing and RNA sequencing, followed by bioinformatics analysis. mA-related RNAs were immunoprecipitated and validated by quantitative real-time PCR (qPCR). In total, 6919 new mA peaks appeared with the disappearance of 5020 peaks in ccRCC samples. The unique mA-related genes in ccRCC were associated with cancer-related pathways. We identified differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated mA peaks in ccRCC. This study presented the first mA transcriptome-wide map of human ccRCC, which may shed lights on possible mechanisms of mA-mediated gene expression regulation.