Chen Yimeng, Zhou Cuixing, Sun Yangyang, He Xiaozhou, Xue Dong
Department of Urology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu, China.
Epigenomics. 2020 Jan;12(2):87-99. doi: 10.2217/epi-2019-0182. Epub 2019 Dec 20.
To systematically profile the global mA modification pattern in clear cell renal cell carcinoma (ccRCC). mA modification patterns in ccRCC and normal tissues were described via mA sequencing and RNA sequencing, followed by bioinformatics analysis. mA-related RNAs were immunoprecipitated and validated by quantitative real-time PCR (qPCR). In total, 6919 new mA peaks appeared with the disappearance of 5020 peaks in ccRCC samples. The unique mA-related genes in ccRCC were associated with cancer-related pathways. We identified differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated mA peaks in ccRCC. This study presented the first mA transcriptome-wide map of human ccRCC, which may shed lights on possible mechanisms of mA-mediated gene expression regulation.
为系统地描绘透明细胞肾细胞癌（ccRCC）中的全局mA修饰模式。通过mA测序和RNA测序描述ccRCC和正常组织中的mA修饰模式，随后进行生物信息学分析。对与mA相关的RNA进行免疫沉淀，并通过定量实时PCR（qPCR）进行验证。在ccRCC样本中，总共出现了6919个新的mA峰，同时有5020个峰消失。ccRCC中独特的与mA相关的基因与癌症相关通路有关。我们在ccRCC中鉴定出具有高甲基化或低甲基化mA峰的差异表达mRNA转录本。本研究展示了首张人类ccRCC的全转录组mA图谱，这可能为mA介导的基因表达调控的潜在机制提供线索。
