mA RNA修饰调节肥厚性瘢痕中的基因表达和纤维化相关通路。
The mA RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar.
作者信息
Liu Si-Yu, Wu Jun-Jie, Chen Zhong-Hua, Zou Ming-Li, Teng Ying-Ying, Zhang Kai-Wen, Li Yue-Yue, Guo Dang-Yang, Yuan Feng-Lai
机构信息
Department of Medicine, Institute of Integrated Traditional Chinese and Western Medicine, Wuxi Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, Wuxi, China.
Institute of Integrated Chinese and Western Medicine, The Hospital Affiliated to Jiangnan University, Wuxi, China.
出版信息
Front Cell Dev Biol. 2021 Nov 15;9:748703. doi: 10.3389/fcell.2021.748703. eCollection 2021.
To systematically analyze the overall mA modification pattern in hyperplastic scars (HS). The mA modification patterns in HS and normal skin (NS) tissues were described by mA sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The mA-related RNA was immunoprecipitated and verified by real-time quantitative PCR. The appearance of 14,791 new mA peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique mA-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated mA peaks. This study is the first to map the mA transcriptome of human HS, which may help clarify the possible mechanism of mA-mediated gene expression regulation.
为系统分析增生性瘢痕（HS）中的整体N⁶-甲基腺嘌呤（mA）修饰模式。通过mA测序和RNA测序描述HS和正常皮肤（NS）组织中的mA修饰模式，随后进行生物信息学分析。对与mA相关的RNA进行免疫沉淀，并通过实时定量PCR进行验证。HS样本中出现14791个新的mA峰，同时有7835个峰消失。因此，HS中独特的与mA相关的基因与纤维化相关途径有关。我们在具有高甲基化或低甲基化mA峰的HS样本中鉴定出差异表达的mRNA转录本。本研究首次绘制了人类HS的mA转录组图谱，这可能有助于阐明mA介导的基因表达调控的可能机制。
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