Campo César Hernán, Martínez María Fernanda, Otero Juan Carlos, Rincón Giovanna
Departamento de Ginecología y Obstetricia, Hospital Universitario de Santander, Bucaramanga, Colombia.
Biomedica. 2019 Dec 1;39(4):689-698. doi: 10.7705/biomedica.4514.
Introduction: Streptococcus agalactiae is the main etiological agent causing invasive infection of the newborn with symptoms that may be associated with septicemia, pneumonia or meningitis and prevalences up to 50% worldwide where there is an increase in antibiotic resistance. Objective: To estimate the prevalence of vagino-rectal colonization by S. agalactiae and its sensitivity profile in pregnant women attending a third-level hospital. Materials and methods: One hundred and twenty one pregnant women were sampled by vaginal and rectal swabs. The cultures were carried out following the methodology recommended by the CDC, and chromID Strepto B agar was added. The suggestive colonies were identified biochemically and the sensitivity profiles according to CLSI were determined. As control, S. pneumoniae ATCC 49619 and S. agalactiae ATCC 12403 were used. Results: Pregnant colonization prevalence by S. agalactiae was 20.66%, with a total of 40 isolates of which 12.5% were non-sensitivity to penicillin. Sensitivity to levofloxacin, clindamycin and erythromycin was 100%, 92.5% y 87.5%, respectively, with the phenotypes iMLSB (3/40) and M (2/40). No sensitivity to tetracycline was found. Conclusions: The prevalence of vagino-rectal colonization by S. agalactiae in the study population was 20.66%, obtaining isolates not sensitive to penicillin and resistance to macrolides and lincosamidas by the Kirby-Bauer technique, so the importance of carrying out active active in pregnant women colonized by Streptococcus agalactiae and to perform constant epidemiological surveillance to detect changes in the sensitivity profiles of the isolates.
无乳链球菌是导致新生儿侵袭性感染的主要病原体，其症状可能与败血症、肺炎或脑膜炎有关，在全球抗生素耐药性增加的地区，患病率高达50%。目的：评估一家三级医院就诊孕妇中无乳链球菌阴道-直肠定植的患病率及其药敏谱。材料和方法：对121名孕妇进行阴道和直肠拭子采样。按照美国疾病控制与预防中心推荐的方法进行培养，并添加chromID B族链球菌琼脂。通过生化方法鉴定可疑菌落，并根据临床和实验室标准协会（CLSI）确定药敏谱。以肺炎链球菌ATCC 49619和无乳链球菌ATCC 12403作为对照。结果：孕妇中无乳链球菌定植患病率为20.66%，共分离出40株菌株，其中12.5%对青霉素不敏感。对左氧氟沙星、克林霉素和红霉素的敏感性分别为100%、92.5%和87.5%，表型为iMLSB（3/40）和M（2/40）。未发现对四环素敏感的菌株。结论：研究人群中无乳链球菌阴道-直肠定植患病率为20.66%，通过 Kirby-Bauer 技术获得了对青霉素不敏感以及对大环内酯类和林可酰胺类耐药的菌株，因此对无乳链球菌定植的孕妇开展积极监测并持续进行流行病学监测以检测分离株药敏谱变化具有重要意义。
