Maehara Y, Kusumoto T, Kusumoto H, Anai H, Sugimachi K
Cancer Center, Kyushu University Hospital, Fukuoka, Japan.
Oncology. 1988;45(6):434-6. doi: 10.1159/000226660.
We compared the colorimetric reactions between the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl 2H-tetrazolium bromide (MTT) assay and the succinate dehydrogenase inhibition (SDI) test, in order to evaluate the usefulness of the SDI test for in vitro chemosensitivity testing. The addition of sodium succinate enhanced the colorimetric absorbance at 565 nm in the MTT assay in a dose- and a time-dependent manner, in mouse sarcoma-180 (S-180) cells. At 10 microM of sodium succinate, a dose used in the SDI test, the absorbance of the MTT assay increased by about 2.5-fold in the S-180 cells and in 10 human tumor tissues. The absorbance in the SDI test correlated well with the viable cell number of S-180 cells (r = 0.9993). These results show that the SDI test, using MTT as a tetrazolium salt, has a higher sensitivity for predicting cell viability, compared to the MTT assay.
我们比较了3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四氮唑蓝（MTT）法与琥珀酸脱氢酶抑制（SDI）试验之间的比色反应，以评估SDI试验用于体外化学敏感性测试的实用性。在小鼠肉瘤-180（S-180）细胞中，添加琥珀酸钠以剂量和时间依赖性方式增强了MTT法在565nm处的比色吸光度。在SDI试验中使用的10μM琥珀酸钠剂量下，MTT法在S-180细胞和10个人类肿瘤组织中的吸光度增加了约2.5倍。SDI试验中的吸光度与S-180细胞的活细胞数密切相关（r = 0.9993）。这些结果表明，与MTT法相比，使用MTT作为四氮唑盐的SDI试验在预测细胞活力方面具有更高的灵敏度。
