Yamaue H, Tanimura H, Tsunoda T, Tani M, Iwahashi M, Noguchi K, Tamai M, Hotta T, Arii K
Department of Gastroenterological Surgery, Wakayama Medical College, Japan.
Eur J Cancer. 1991;27(10):1258-63. doi: 10.1016/0277-5379(91)90093-s.
A major problem associated with the succinate dehydrogenase inhibition (SDI) test using tetrazolium dye (MTT) as a cancer chemosensitivity testing is the contamination of non-malignant cells in the tumour tissues. Highly purified fresh human tumour cells from 44 solid tumours and 24 malignant ascites were used for the MTT assay. The purity of tumour cells was greater than 90% after separation on Ficoll-Hypaque and Percoll discontinuous gradients. The OD570 obtained from tumour cells alone was higher than that from non-malignant cells. The chemosensitivity of tumour cells was distinct from that of non-malignant cells. Moreover, the chemosensitivity of highly purified tumour cells was also distinct from that of non-purified cells just separated from tumour tissues. 31 of the 68 patients had evaluable lesions, and received cancer chemotherapy according to the results of MTT assay using highly purified tumour cells. A clinical response was obtained in 10 of the 31 patients (response rate = 32.3%, 5 complete responses, 5 partial responses).
使用四氮唑染料（MTT）进行琥珀酸脱氢酶抑制（SDI）试验作为癌症化学敏感性测试的一个主要问题是肿瘤组织中非恶性细胞的污染。来自44例实体瘤和24例恶性腹水的高度纯化的新鲜人肿瘤细胞用于MTT试验。在Ficoll-Hypaque和Percoll不连续梯度上分离后，肿瘤细胞的纯度大于90%。仅从肿瘤细胞获得的OD570高于非恶性细胞。肿瘤细胞的化学敏感性与非恶性细胞不同。此外，高度纯化的肿瘤细胞的化学敏感性也与刚从肿瘤组织中分离出的未纯化细胞不同。68例患者中有31例有可评估病变，并根据使用高度纯化肿瘤细胞的MTT试验结果接受癌症化疗。31例患者中有10例获得临床缓解（缓解率=32.3%，5例完全缓解，5例部分缓解）。
