Costagliola C, Iuliano G, Menzione M, Nesti A, Simonelli F, Rinaldi E
Eye Clinic, 1st School of Medicine, University of Naples, Italy.
Ophthalmic Res. 1988;20(5):308-16. doi: 10.1159/000266734.
In this study we have investigated the oxidative metabolism of red blood cells (RBC), plasma, serum, aqueous humor, and lens of healthy subjects and of age-matched cataractous patients with and without diabetes. Reduced and oxidized glutathione (GSH GSSG) levels in RBC were similar among the three groups. Plasma levels of GSSG were higher in diabetics than in cataractous and control subjects. No differences in plasma content of GSH were noted among the three groups. The activity of the enzyme glucose-6-phosphate dehydrogenase was significantly diminished in diabetic patients. Controls and cataractous patients showed similar levels of malondialdehyde (MDA). Although not significant the MDA content in RBC from diabetics was elevated. No differences in plasma levels of vitamin E were noted among the three groups. The biological liquid oxidant activity of serum in diabetic patients was significantly higher than in controls and cataractous patients. GSH levels in aqueous humor were similar in diabetic and nondiabetic cataractous patients. The content of GSSG in aqueous humor was highest in diabetic patients. Control clear lenses showed low levels of MDA. The MDA levels in cataractous lenses from nondiabetic patients were significantly higher than those of controls. In diabetic patients the content of MDA in the lens was approximately twice as high as the cataractous values. Our results seem to demonstrate that oxidative damage could play a role in the pathogenesis of cataract in diabetes.
在本研究中，我们调查了健康受试者以及年龄匹配的有糖尿病和无糖尿病的白内障患者的红细胞（RBC）、血浆、血清、房水和晶状体的氧化代谢。三组受试者红细胞中还原型和氧化型谷胱甘肽（GSH、GSSG）水平相似。糖尿病患者血浆中GSSG水平高于白内障患者和对照组受试者。三组受试者血浆中GSH含量无差异。糖尿病患者中葡萄糖-6-磷酸脱氢酶的活性显著降低。对照组和白内障患者丙二醛（MDA）水平相似。尽管差异不显著，但糖尿病患者红细胞中MDA含量升高。三组受试者血浆中维生素E水平无差异。糖尿病患者血清的生物液体氧化活性显著高于对照组和白内障患者。糖尿病和非糖尿病白内障患者房水中GSH水平相似。糖尿病患者房水中GSSG含量最高。对照的透明晶状体MDA水平较低。非糖尿病患者白内障晶状体中MDA水平显著高于对照组。糖尿病患者晶状体中MDA含量约为白内障患者的两倍。我们的结果似乎表明氧化损伤可能在糖尿病性白内障的发病机制中起作用。
