Costagliola C, Iuliano G, Menzione M, Simonelli F, Tortori A, Masturzi B, di Benedetto A, Rinaldi E
Eye Clinic, 1st School of Medicine, University of Naples, Italy.
Exp Eye Res. 1990 Dec;51(6):631-5. doi: 10.1016/0014-4835(90)90046-w.
In this study we have investigated the oxidative metabolism of red cells (RBC), plasma, serum and aqueous humour of healthy subjects and of age-matched cataractous patients with and without chronic renal failure (CRF). Reduced glutathione (GSH) levels in RBC were lower in CRF patients than in the other groups. Oxidized glutathione (GSSG) plasma levels in CRF patients were higher than those of controls and cataractous subjects. The activity of the enzyme glucose-6-phosphate dehydrogenase in RBC was significantly reduced in CRF patients with respect to the other two groups. The levels of malondialdehyde (MDA) in RBC and in lens were about twice in CRF patients compared with the other two groups. The plasma levels of vitamin E were diminished in CRF patients; on the contrary, the biological liquid oxidant activity (BLOA) of serum in CRF patients was significantly higher than in controls and in cataractous patients without CRF. Cataractous patients with and without CRF showed similar levels of GSH in aqueous humour; on the contrary, the content of GSSG was significantly higher in CRF patients. Our findings seem to demonstrate that CRF patients are exposed to oxidative stresses that could probably act synergistically with uraemia and carbamylation of lens proteins. This synergism could explain why CRF represents a relatively high risk factor for cataract.
在本研究中，我们调查了健康受试者以及年龄匹配的患有和未患有慢性肾衰竭（CRF）的白内障患者的红细胞（RBC）、血浆、血清和房水的氧化代谢情况。CRF患者红细胞中的还原型谷胱甘肽（GSH）水平低于其他组。CRF患者血浆中的氧化型谷胱甘肽（GSSG）水平高于对照组和白内障患者。与其他两组相比，CRF患者红细胞中葡萄糖-6-磷酸脱氢酶的活性显著降低。与其他两组相比，CRF患者红细胞和晶状体中的丙二醛（MDA）水平约为其两倍。CRF患者血浆中的维生素E水平降低；相反，CRF患者血清的生物液体氧化活性（BLOA）显著高于对照组和无CRF的白内障患者。患有和未患有CRF的白内障患者房水中的GSH水平相似；相反，CRF患者中GSSG的含量显著更高。我们的研究结果似乎表明，CRF患者面临氧化应激，这种应激可能与尿毒症和晶状体蛋白的氨甲酰化协同作用。这种协同作用可以解释为什么CRF是白内障的一个相对较高的危险因素。
