Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey.
Burns. 2020 Feb;46(1):143-155. doi: 10.1016/j.burns.2019.02.013. Epub 2019 Dec 18.
Electrospinning process has gained importance in the production of wound dressings in recent years. The wound dressings prepared by electrospinning method provide many advantages over conventional wound dressings. The aim of this study was to assess the histological, biochemical, and immunohistochemical evaluation of collagen/doxycycline loaded nanofiber wound dressing in both acute and chronic wound healing. Full-thickness wound model was created on rats and rats were divided in two main groups: normoglycemic (acute) and hyperglycemic (chronic) groups. Each group was divided into three sub groups: not treated (control) group, treated with nanofiber wound dressing group and treated with commercial product group. Wound closure rates were measured. Oxidative events were investigated by biochemical analyses. In addition to histological studies, matrix metalloproteinase, tissue inhibitor of metalloproteinase, vascular endothelial growth factor, basic-fibroblast growth factor, and von Willebrand factor levels were investigated with immunohistochemical studies. According to the biochemical analyses, it was concluded that the nanofiber wound dressing helps to increase antioxidant capacity and decrease lipid peroxidation. Immunohistochemical studies showed that nanofiber wound dressing enhanced angiogenesis and shortened the inflammatory phase. It was concluded that an effective and safe prototype nanofiber wound dressing, which has similar wound healing effect to the commercial product, has been developed to be used in acute or chronic wound treatment.
近年来，电纺工艺在创伤敷料的生产中得到了重视。电纺法制备的创伤敷料相对于传统的创伤敷料具有许多优势。本研究旨在评估胶原/强力霉素负载纳米纤维创伤敷料在急性和慢性创伤愈合中的组织学、生化和免疫组织化学评价。在大鼠上创建全层创面模型，并将大鼠分为两个主要组：正常血糖（急性）和高血糖（慢性）组。每个组又分为三个亚组：未治疗（对照）组、纳米纤维创伤敷料治疗组和商业产品治疗组。测量创面闭合率。通过生化分析研究氧化事件。除了组织学研究外，还通过免疫组织化学研究研究了基质金属蛋白酶、金属蛋白酶组织抑制剂、血管内皮生长因子、碱性成纤维细胞生长因子和血管性血友病因子的水平。根据生化分析的结果，可以得出结论纳米纤维创伤敷料有助于提高抗氧化能力并减少脂质过氧化。免疫组织化学研究表明，纳米纤维创伤敷料促进了血管生成并缩短了炎症期。结论是已经开发出一种有效且安全的原型纳米纤维创伤敷料，其具有类似于商业产品的创伤愈合效果，可用于急性或慢性伤口治疗。
