Grosu Oxana-Madalina, Dragostin Oana-Maria, Gardikiotis Ioannis, Chitescu Carmen Lidia, Lisa Elena Lacramioara, Zamfir Alexandra-Simona, Confederat Luminita, Dragostin Ionut, Dragan Maria, Stan Catalina Daniela, Zamfir Carmen-Lacramioara
Department of Surgery I, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania.
Research Centre in the Medical-Pharmaceutical Field, Faculty of Medicine and Pharmacy, "Dunarea de Jos" University of Galati, 47 Domneasca Street, 800008 Galati, Romania.
Biomedicines. 2023 Mar 10;11(3):852. doi: 10.3390/biomedicines11030852.
Considering that microbial resistance to antibiotics is becoming an increasingly widespread problem, burn management, which usually includes the use of topical antimicrobial dressings, is still facing difficulties regarding their efficiency to ensure rapid healing. In this context, the main objective of this research is to include new oxytetracycline derivatives in polymeric-film-type dressings for the treatment of wounds caused by experimentally induced burns in rats. The structural and physico-chemical properties of synthesized oxytetracycline derivatives and the corresponding membranes were analyzed by FT-IR and MS spectroscopy, swelling ability and biodegradation capacity. In vitro antimicrobial activity using Gram-positive and Gram-negative bacterial strains and pathogenic yeasts, along with an in vivo study of a burn wound model induced in Wistar rats, was also analyzed. The newly obtained polymeric films, namely chitosan-oxytetracycline derivative membranes, showed good antimicrobial activity noticed in the tested strains, a membrane swelling ratio (MSR) of up to 1578% in acidic conditions and a biodegradation rate of up to 15.7% on day 7 of testing, which are important required characteristics for the tissue regeneration process, after the production of a burn. The in vivo study proved that chitosan-derived oxytetracycline membranes showed also improved healing effects which contributes to supporting the idea of using them for the treatment of wounds caused by burns.
鉴于微生物对抗生素的耐药性正成为一个日益普遍的问题，烧伤处理通常包括使用局部抗菌敷料，但在确保快速愈合的效率方面仍面临困难。在此背景下，本研究的主要目的是将新的土霉素衍生物纳入聚合物膜型敷料中，用于治疗大鼠实验性诱导烧伤所致的伤口。通过傅里叶变换红外光谱（FT-IR）和质谱（MS）光谱、溶胀能力和生物降解能力分析了合成的土霉素衍生物及相应膜的结构和物理化学性质。还分析了使用革兰氏阳性和革兰氏阴性细菌菌株以及致病性酵母的体外抗菌活性，以及对Wistar大鼠诱导的烧伤伤口模型的体内研究。新获得的聚合物膜，即壳聚糖-土霉素衍生物膜，在测试菌株中显示出良好的抗菌活性，在酸性条件下膜溶胀率（MSR）高达1578%，在测试第7天生物降解率高达15.7%，这些都是烧伤后组织再生过程所需的重要特性。体内研究证明，壳聚糖衍生的土霉素膜也显示出改善的愈合效果，这有助于支持将其用于治疗烧伤所致伤口的观点。
