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DNA 回收对随后潜在指印质量的影响:一项模拟操作试验。

The effect of DNA recovery on the subsequent quality of latent fingermarks: A pseudo-operational trial.

机构信息

School of Law, Policing and Forensics, Staffordshire University, Stoke-on-Trent, England, ST42DF, United Kingdom.

School of Applied Science, London South Bank University, 103 Borough Road, London SE1 0AA, United Kingdom.

出版信息

Forensic Sci Int. 2020 Feb;307:110076. doi: 10.1016/j.forsciint.2019.110076. Epub 2019 Nov 26.

Abstract

The recovery of fingermarks and DNA from the same location at a crime scene can be problematic because of contamination issues associated with powdering or laboratory-based visualisation processes and/or the perceived destructive impact of commonly employed 'swabbing' approaches to DNA recovery. Previous research in a controlled environment demonstrated that it was possible to recover DNA and latent fingermarks from the same location on various substrates when an adhesive approach to DNA recovery was used. The aim of this research was to conduct a pseudo-operational trial into the dual recovery of DNA and fingermarks using gel lifters for DNA recovery. Participants were asked to voluntarily and anonymously donate a wide variety of porous and non-porous substrates post handling. No instruction as to fingermark deposition nor environmental storage was provided. BVDA gel lifters were applied to the substrates to replicate DNA recovery followed by the application of fingermark visualisation processes. The number and quality of the fingermarks was established using a grading approach. Application factors were also investigated to consider the effects of user variation. The results demonstrated that it was possible to recover DNA and fingermarks considered to be capable of supporting an identification. Fingermark quality post lifting was dependant on the substrates used. The weight applied to the gel during its application was a lesser contributing factor than the duration of its contact with the surface. There was a greater chance of leaving the fingermarks unaltered with the application of a low weight and instantaneous retraction.

摘要

从犯罪现场的同一地点恢复指纹和 DNA 可能会出现问题,因为粉末化或基于实验室的可视化过程以及/或通常采用的 DNA 恢复“擦拭”方法所带来的破坏性影响而存在污染问题。先前在受控环境中的研究表明,当使用粘性 DNA 恢复方法时,可以从各种基质上的同一位置恢复 DNA 和潜在指纹。本研究的目的是使用凝胶提取器进行 DNA 恢复的双恢复进行伪操作试验。要求参与者在处理后自愿匿名捐献各种多孔和非多孔基质。没有关于指纹沉积或环境储存的说明。将 BVDA 凝胶提取器应用于基质以复制 DNA 恢复,然后应用指纹可视化过程。使用分级方法确定指纹的数量和质量。还研究了应用因素以考虑用户变化的影响。结果表明,有可能恢复被认为能够支持鉴定的 DNA 和指纹。提起后的指纹质量取决于所使用的基质。在施加凝胶时的重量比其与表面接触的时间的影响要小。用低重量和瞬时缩回施加凝胶时,更有可能使指纹保持不变。

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