Kwok Ross, Parsons Robin, Fieldhouse Sarah, Walton-Williams Laura
School of Justice, Security and Sustainability, Staffordshire University, ST42DF, UK.
Forensic Sci Int. 2023 Mar;344:111574. doi: 10.1016/j.forsciint.2023.111574. Epub 2023 Jan 25.
Dual recovery of forensic evidence is beneficial for crime scene and evidence processing as it can potentially double the evidential value of a single source, even more so in instances of DNA fingermarks. The use of adhesive liftering media has shown comparable results to swabbing when dealing with trace DNA recovery. Gelatine lifters have displayed the potential to recover DNA from latent fingermarks with minimal alteration to friction ridge detail post application, yet their ability to recover DNA has not fully been explored. The aim of this research was to compare the use of gelatine lifters with more readily available masking tape in their ability to recover cellular material from latent fingermarks. Natural (n = 120) and sebaceous (n = 120) fingermarks were deposited and aged in time frames from fresh, 1-day, 2-day, 1-week, 2-weeks, and 1 month. DiamondTM Nucleic Acid Dye was used as a visualisation method for any DNA containing cellular material. Images of the fingermarks pre and post lifting, and on the lifting media were imaged using the DSC®5 system. The media's ability to recover cellular material was assessed using fluorescent particle analysis by the employment of the free software ImageJ. Fluorescent particles could be observed on the lifting media post lifting with the use of DiamondTM Dye. Time was not seen to influence the variation in the number of fluorescent particles observed. The use of gelatine lifters was found to have a higher amount of recovered DNA containing cellular material than masking tape. Visualisation of particles on masking tape were inhibited by its porosity and absorption of the dye. Some fingermark detail could be observed in the gelatine lifters. The DSC®5 system was suitable for imaging fingermarks stained with DiamondTM Dye.
法医证据的双重回收对犯罪现场和证据处理有益，因为它有可能使单一来源的证据价值翻倍，在DNA指纹的情况下更是如此。在处理微量DNA回收时，使用粘性提取介质已显示出与擦拭相当的结果。明胶提取器已显示出从潜在指纹中回收DNA的潜力，在应用后对摩擦嵴细节的改变最小，但它们回收DNA的能力尚未得到充分探索。本研究的目的是比较明胶提取器与更容易获得的遮蔽胶带从潜在指纹中回收细胞物质的能力。自然（n = 120）和皮脂腺（n = 120）指纹在新鲜、1天、2天、1周、2周和1个月的时间范围内沉积并老化。DiamondTM核酸染料用作任何含DNA细胞物质的可视化方法。使用DSC®5系统对提取前后的指纹以及提取介质上的指纹进行成像。通过使用免费软件ImageJ进行荧光颗粒分析来评估介质回收细胞物质的能力。使用DiamondTM染料提取后，可以在提取介质上观察到荧光颗粒。时间并未被发现会影响观察到的荧光颗粒数量的变化。发现使用明胶提取器比遮蔽胶带回收的含DNA细胞物质更多。遮蔽胶带上颗粒的可视化受到其孔隙率和染料吸收的抑制。在明胶提取器中可以观察到一些指纹细节。DSC®5系统适用于对用DiamondTM染料染色的指纹进行成像。
