Hu Xueyan, Xu Jie, Li Wenfang, Li Liying, Parungao Roxanne, Wang Yiwei, Zheng Shuangshuang, Nie Yi, Liu Tianqing, Song Kedong
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China.
Key Laboratory of Biological Medicines, Universities of Shandong Province Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, China.
Appl Biochem Biotechnol. 2020 Jun;191(2):785-809. doi: 10.1007/s12010-019-03214-8. Epub 2019 Dec 21.
Repairing osteochondral defects to restore joint function is a major challenge in regenerative medicine. However, with recent advances in tissue engineering, the development of potential treatments is promising. In recent years, in addition to single-layer scaffolds, double-layer or multilayer scaffolds have been prepared to mimic the structure of articular cartilage and subchondral bone for osteochondral repair. Although there are a range of different cells such as umbilical cord stem cells, bone marrow mesenchyml stem cell, and others that can be used, the availability, ease of preparation, and the osteogenic and chondrogenic capacity of these cells are important factors that will influence its selection for tissue engineering. Furthermore, appropriate cell proliferation and differentiation of these cells is also key for the optimal repair of osteochondral defects. The development of bioreactors has enhanced methods to stimulate the proliferation and differentiation of cells. In this review, we summarize the recent advances in tissue engineering, including the development of layered scaffolds, cells, and bioreactors that have changed the approach towards the development of novel treatments for osteochondral repair.
修复骨软骨缺损以恢复关节功能是再生医学中的一项重大挑战。然而，随着组织工程学的最新进展，潜在治疗方法的开发前景广阔。近年来，除了单层支架外，还制备了双层或多层支架，以模拟关节软骨和软骨下骨的结构用于骨软骨修复。尽管有多种不同的细胞，如脐带干细胞、骨髓间充质干细胞等可用于此，但这些细胞的可用性、制备的难易程度以及成骨和成软骨能力是影响其在组织工程中选择的重要因素。此外，这些细胞的适当增殖和分化也是骨软骨缺损最佳修复的关键。生物反应器的发展改进了刺激细胞增殖和分化的方法。在这篇综述中，我们总结了组织工程学的最新进展，包括分层支架、细胞和生物反应器的发展，这些进展改变了骨软骨修复新治疗方法的开发途径。
