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磷酸酶与张力蛋白同源物（PTEN）、钙黏蛋白 1（CDH1）和钙黏蛋白 2（CDH2）基因及细胞膜蛋白 CD133 在人子宫内膜腺癌 Ishikawa 细胞系中的表达谱。

Expression Profiles of the Phosphatase and Tensin Homolog (PTEN), CDH1, and CDH2 Genes, and the Cell Membrane Protein, CD133, in the Ishikawa Human Endometrial Adenocarcinoma Cell Line.

机构信息

Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China (mainland).

出版信息

Med Sci Monit. 2019 Dec 21;25:9829-9835. doi: 10.12659/MSM.918787.

DOI:10.12659/MSM.918787
PMID:31864232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6937906/
Abstract

BACKGROUND This study aimed to investigate the expression profile of the phosphatase and tensin homolog (PTEN) gene, the cadherin genes, CDH1 and CDH2, and the cell membrane glycoprotein, CD133, in the Ishikawa human endometrial adenocarcinoma cell line. MATERIAL AND METHODS The Ishikawa endometrial carcinoma cell groups included cells transfected with the pLVX-puro lentiviral expression vector (the Ishikawa-puro group) and cells transfected with the pLVX-puro-PTEN lentiviral expression vector (the Ishikawa-PTEN group). The mRNA expression of the cadherin genes, CDH1 and CDH2, was detected by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The expression levels of the transmembrane glycoprotein CD133, a cancer stem cell marker, was detected by flow cytometry. RESULTS The expression of CDH1 and CDH2 mRNA in the Ishikawa-PTEN cells was lower than in the control cells. CD133 expression was lower in the Ishikawa-PTEN cells compared with the control cells. CONCLUSIONS This in vitro study showed that in Ishikawa endometrial carcinoma cells, downregulation of PTEN was associated with the expression of the CDH1 and CDH2 genes and upregulated expression of the cell membrane glycoprotein, CD133, which are associated with epithelial-mesenchymal transition (EMT) in malignancy. These findings support the need for further studies to investigate the potential role of PTEN in invasion and metastasis in endometrial carcinoma.

摘要

背景

本研究旨在探讨磷酸酶和张力蛋白同源物（PTEN）基因、钙粘蛋白基因 CDH1 和 CDH2 以及细胞膜糖蛋白 CD133 在人子宫内膜腺癌 Ishikawa 细胞系中的表达谱。

材料与方法

Ishikawa 子宫内膜癌细胞组包括转染 pLVX-puro 慢病毒表达载体的细胞（Ishikawa-puro 组）和转染 pLVX-puro-PTEN 慢病毒表达载体的细胞（Ishikawa-PTEN 组）。采用实时定量逆转录聚合酶链反应（RT-qPCR）检测钙粘蛋白基因 CDH1 和 CDH2 的 mRNA 表达。采用流式细胞术检测跨膜糖蛋白 CD133 的表达水平，CD133 是一种癌症干细胞标志物。

结果

Ishikawa-PTEN 细胞中 CDH1 和 CDH2 mRNA 的表达低于对照细胞。Ishikawa-PTEN 细胞中 CD133 的表达低于对照细胞。

结论

这项体外研究表明，在 Ishikawa 子宫内膜癌细胞中，PTEN 的下调与 CDH1 和 CDH2 基因的表达以及细胞膜糖蛋白 CD133 的上调有关，这与恶性肿瘤中的上皮-间充质转化（EMT）有关。这些发现支持进一步研究 PTEN 在子宫内膜癌侵袭和转移中的潜在作用的必要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1298/6937906/9d7ec4f487ff/medscimonit-25-9829-g004.jpg
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