National Institute of Fisheries Science, West Sea Fisheries Research Institute, Fisheries Research & Devlopment, Taean, 32132, South Korea.
National Institute of Fisheries Science, West Sea Fisheries Research Institute, Fisheries Research & Devlopment, Taean, 32132, South Korea.
Fish Shellfish Immunol. 2020 Feb;97:581-586. doi: 10.1016/j.fsi.2019.12.059. Epub 2019 Dec 19.
Paralichthys olivaceus (mean weight, 280.1 ± 10.5 g; mean length, 28.37 ± 2.3 cm) was reared in bio-floc and seawater for 6 months to determine the toxic effects of waterborne nitrite exposure (0, 25, 50, 100, and 200 mg/L) for 1 week, compared to those observed with bio-floc and seawater only. The effects on antioxidant activity, immune responses, and acetylcholinesterase activity were measured. Following nitrite exposure, superoxide dismutase activity in the liver and gills was significantly elevated and catalase activity was significantly increased, except for in the gills of P. olivaceus reared in bio-floc. Further, glutathione S-transferase activity was significantly elevated in the liver and gills, and glutathione was significantly lower. Meanwhile, acetylcholinesterase activity in the liver and gills was significantly inhibited and plasma lysozyme activity and immunoglobulin M were considerably elevated.
牙鲆（平均体重 280.1±10.5 克，平均体长 28.37±2.3 厘米）在生物絮团和海水中养殖 6 个月，以确定在为期 1 周的时间内，与仅在生物絮团和海水中养殖相比，水中亚硝酸盐暴露（0、25、50、100 和 200mg/L）的毒性影响。测量了抗氧化活性、免疫反应和乙酰胆碱酯酶活性的变化。在亚硝酸盐暴露后，牙鲆肝脏和鳃中超氧化物歧化酶活性显著升高，过氧化氢酶活性显著升高，但生物絮团养殖的牙鲆除外。此外，肝脏和鳃中的谷胱甘肽 S-转移酶活性显著升高，谷胱甘肽显著降低。同时，肝脏和鳃中的乙酰胆碱酯酶活性显著受到抑制，血浆溶菌酶活性和免疫球蛋白 M 显著升高。
