Chen Sheng, Tang Yuhang, Zhang Zhou, Zheng Jichang, He Yuliang, Wang Zhen, Mai Kangsen, Ai Qinghui
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Rural Affairs, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, China.
Aquac Nutr. 2022 Sep 22;2022:8529556. doi: 10.1155/2022/8529556. eCollection 2022.
A 70-day feeding experiment was carried out to assess the replacement of dietary fishmeal (FM) protein with degossypolized cottonseed protein (DCP) on large yellow croaker () with initial body weight (13.09 ± 0.50 g). Five isonitrogenous and isolipidic diets replaced fishmeal protein with 0%, 20%, 40%, 60%, and 80% DCP were formulated and named as FM (the control group), DCP20, DCP40, DCP60, and DCP80, respectively. Results displayed that weight gain rate (WGR) and specific growth rate (SGR) in the DCP20 group (263.91% and 1.85% d) were significantly increased compared with the control group (194.79% and 1.54% d) ( < 0.05). Furthermore, fish fed the diet with 20% DCP significantly increased the activity of hepatic superoxide dismutase (SOD) compared with the control group ( < 0.05). Meanwhile, the content of hepatic malondialdehyde (MDA) in the DCP20, DCP40, and DCP80 groups was significantly lower than that in the control group ( < 0.05). The activity of intestinal trypsin in the DCP20 group was significantly degraded compared with that in the control group ( < 0.05). The transcription of hepatic proinflammatory cytokine genes (interleukin-6 (); tumor necrosis factor- (); and interferon- ()) in the DCP20 and DCP40 groups was significantly upregulated compared with that in the control group ( < 0.05). As to the target of rapamycin (TOR) pathway, the transcription of hepatic target of rapamycin () and ribosomal protein () was significantly up-regulated, while the transcription of hepatic eukaryotic translation initiation factor 4E binding protein 1 () gene was significantly downregulated in the DCP group compared with the control group ( < 0.05). In summary, based on the broken line regression model analysis of WGR and SGR against dietary DCP replacement levels, the optimal replacement level was recommended to be 8.12% and 9.37% for large yellow croaker, respectively. These results revealed that FM protein replaced with 20% DCP could promote digestive enzyme activities and antioxidant capacity and further activate immune response and the TOR pathway so that growth performance of juvenile large yellow croaker was improved.
进行了一项为期70天的饲养实验，以评估用脱棉酚棉籽蛋白（DCP）替代大黄鱼（初始体重为13.09±0.50克）日粮中的鱼粉（FM）蛋白的效果。配制了五种等氮等脂日粮，分别用0%、20%、40%、60%和80%的DCP替代鱼粉蛋白，并分别命名为FM（对照组）、DCP20、DCP40、DCP60和DCP80。结果显示，DCP20组的增重率（WGR）和特定生长率（SGR）（分别为263.91%和1.85%/天）与对照组（194.79%和1.54%/天）相比显著提高（P<0.05）。此外，与对照组相比，饲喂含20% DCP日粮的鱼肝脏超氧化物歧化酶（SOD）活性显著提高（P<0.05）。同时，DCP20、DCP40和DCP80组肝脏丙二醛（MDA）含量显著低于对照组（P<0.05）。DCP20组肠道胰蛋白酶活性与对照组相比显著降低（P<0.05）。与对照组相比，DCP20和DCP40组肝脏促炎细胞因子基因（白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ））的转录显著上调（P<0.05）。至于雷帕霉素靶蛋白（TOR）途径，与对照组相比，DCP组肝脏雷帕霉素靶蛋白（TOR）和核糖体蛋白（S6K）的转录显著上调，而肝脏真核翻译起始因子4E结合蛋白1（4E-BP1）基因的转录显著下调（P<0.05）。综上所述，基于WGR和SGR对日粮DCP替代水平的折线回归模型分析，建议大黄鱼的最佳替代水平分别为8.12%和9.37%。这些结果表明，用20% DCP替代FM蛋白可促进消化酶活性和抗氧化能力，并进一步激活免疫反应和TOR途径，从而提高大黄鱼幼鱼的生长性能。
