Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, China.
Biosci Trends. 2020 Jan 20;13(6):530-538. doi: 10.5582/bst.2019.01332. Epub 2019 Dec 21.
Shufengjiedu capsules (SFJDCs), a traditional Chinese medicine, have been widely used as an antiviral, antibacterial, antitumor, and anti-inflammatory drug. However, the roles of SFJDCs in allergic rhinitis remain unclear. The purpose of this study was to investigate the effects of SFJDCs in olfaction and lung injury in rats with allergic rhinitis. An animal model of allergic rhinitis was created by intraperitoneal injection and intranasal administration of ovalbumin to rats. All rats were divided into seven groups: a model group, a low-dose SFJDC group, a medium-dose SFJDC group, a high-dose SFJDC group, a cetirizine group, and a control group. Hematoxylin and eosin (HE) staining was used to observe pathological changes in rat lung and olfactory epithelium (OE) tissue, and peripheral blood was collected and subjected to an enzyme-linked immunosorbent assay (ELISA) to detect IgE, tumor necrosis factor alpha (TNF-α), and IL-1ꞵ levels. Western blotting, immunohistochemistry staining, and immunofluorescence staining were performed to detect inflammatory cytokines and levels of the autophagy biomarker beclin1 and the apoptosis biomarker cleaeved-caspased3 in lung and OE tissue. ELISA indicated that SFJDCs significantly decreased IgE, TNF-α, and IL-1ꞵ levels in peripheral blood, the lungs, and OE tissue. In addition, Western blotting and staining indicated that SFJDCs repair lung injury, protect against neuronal apoptosis in OE, and rescue impaired autophagy in the lungs and OE tissue. In conclusion, results indicated that SFJDCs might protect against neuronal loss in the OE and lung injury by enhancing autophagy and decreasing apoptosis in rats with allergic rhinitis. Therefore, SFJDCs might serve as an alternative treatment for allergic rhinitis.
疏风解毒胶囊（SFJDCs）是一种中药，被广泛用作抗病毒、抗菌、抗肿瘤和抗炎药物。然而，SFJDCs 在过敏性鼻炎中的作用尚不清楚。本研究旨在探讨 SFJDCs 对过敏性鼻炎大鼠嗅觉和肺损伤的影响。通过向大鼠腹腔内注射和鼻腔内给予卵清蛋白来建立过敏性鼻炎动物模型。所有大鼠分为 7 组：模型组、SFJDC 低剂量组、SFJDC 中剂量组、SFJDC 高剂量组、西替利嗪组和对照组。苏木精和伊红（HE）染色用于观察大鼠肺和嗅上皮（OE）组织的病理变化，采集外周血并进行酶联免疫吸附测定（ELISA）以检测 IgE、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。Western blot、免疫组化染色和免疫荧光染色用于检测肺和 OE 组织中的炎症细胞因子以及自噬标志物 beclin1 和凋亡标志物 cleaved-caspased3 的水平。ELISA 结果表明，SFJDCs 可显著降低外周血、肺和 OE 组织中的 IgE、TNF-α和 IL-1β水平。此外，Western blot 和染色结果表明，SFJDCs 可修复肺损伤，保护 OE 中神经元凋亡，并挽救过敏性鼻炎大鼠肺和 OE 组织中受损的自噬。综上所述，SFJDCs 可能通过增强自噬和减少凋亡来保护过敏性鼻炎大鼠的 OE 神经元丢失和肺损伤，因此 SFJDCs 可能成为治疗过敏性鼻炎的一种替代方法。
