Takano M, Rhoads D B, Isselbacher K J
Gastrointestinal Unit, Massachusetts General Hospital, Boston.
Proc Natl Acad Sci U S A. 1988 Nov;85(21):8072-5. doi: 10.1073/pnas.85.21.8072.
The effect of sodium butyrate on the expression of the facilitated glucose transporter (GT) was investigated in the pig kidney cell line LLC-PK1. When cells were treated with butyrate, GT mRNA expression was remarkably enhanced with a maximal effect at 5 mM. Levels of GT mRNA were increased at 1 day after butyrate treatment and continued to increase for at least 4 days; however, acetate and propionate did not affect GT mRNA levels significantly. The induction of GT mRNA by butyrate was accompanied by an increase in GT function. The expression of GT mRNA decreased in HepG2, HT-29, and COS cells by treatment with butyrate for 1 day. Interestingly, glucose deprivation of LLC-PK1 cells reduced the induction of GT mRNA by butyrate, although starvation itself slightly enhanced steady-state GT mRNA levels. Therefore, expression of GT in LLC-PK1 cells is strongly induced by butyrate by a pathway that apparently depends on the presence of glucose in culture medium.
在猪肾细胞系LLC-PK1中研究了丁酸钠对易化葡萄糖转运体（GT）表达的影响。用丁酸盐处理细胞时，GT mRNA表达显著增强，在5 mM时达到最大效应。丁酸盐处理后1天GT mRNA水平升高，并持续至少4天增加；然而，乙酸盐和丙酸盐对GT mRNA水平没有显著影响。丁酸盐对GT mRNA的诱导伴随着GT功能的增加。用丁酸盐处理1天，HepG2、HT-29和COS细胞中GT mRNA的表达降低。有趣的是，LLC-PK1细胞的葡萄糖剥夺降低了丁酸盐对GT mRNA的诱导，尽管饥饿本身略微提高了稳态GT mRNA水平。因此，LLC-PK1细胞中GT的表达通过一条显然依赖于培养基中葡萄糖存在的途径被丁酸盐强烈诱导。
