粪卟啉原III氧化酶的线粒体定位。
The mitochondrial localization of coproporphyrinogen III oxidase.
作者信息
Grandchamp B, Phung N, Nordmann Y
出版信息
Biochem J. 1978 Oct 15;176(1):97-102. doi: 10.1042/bj1760097.
Abstract
The location of coproporphyrinogen III oxidase in mitochondria was studied in rat liver by using the digitonin method or hypo-osmotic media for fractionation. The enzyme was found in the intermembrane space with a fraction loosely bound to the inner membrane. This fraction was released by washing the inner-membrane-matrix complex with alkaline solutions or solutions of high ionic strength. The enzyme in both fractions had the same Km (0.16 micrometer) for coproporphyrinogen III. When incubation was performed in a medium that avoided destruction of enzyme membrane binding, a dramatic increase in activity was observed after sonication of whole mitochondria or of the inner-membrane-matrix complex.
摘要
采用洋地黄皂苷法或低渗介质分级分离法，对大鼠肝脏线粒体中粪卟啉原III氧化酶的定位进行了研究。发现该酶存在于膜间隙，有一部分与内膜松散结合。通过用碱性溶液或高离子强度溶液洗涤内膜-基质复合物可释放出这部分酶。两部分中的酶对粪卟啉原III的Km值相同（0.16微米）。当在避免破坏酶与膜结合的介质中进行孵育时，对完整线粒体或内膜-基质复合物进行超声处理后，酶活性显著增加。
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