Instituto de Salud Pública, Universidad Veracruzana, Veracruz, México.
Programa de Maestría en Ciencias de la Salud, Instituto de Ciencias de la Salud, Universidad Veracruzana, Veracruz, México.
Microb Drug Resist. 2020 Jul;26(7):732-740. doi: 10.1089/mdr.2019.0054. Epub 2019 Dec 23.
Whole genome sequencing (WGS) has been proposed as a tool for the diagnosis of drug resistance in tuberculosis (TB); however, there have been few studies on its effectiveness in countries with significantly high drug resistance rates. This study therefore aimed to evaluate the effectiveness of WGS to identify mutations related to drug resistance in TB isolates from an endemic region of Mexico. The results showed that, of 35 multidrug-resistant isolates analyzed, the values of congruence found between the phenotypic drug susceptibility testing and polymorphisms were 94% for isoniazid, 97% for rifampicin, 90% for ethambutol, and 82% for pyrazinamide. It was also possible to identify eight isolates as potential pre-extensive drug resistant (XDR) and one as XDR. Twenty nine isolates were classified within L4 and two transmission clusters were identified. The results show the potential utility of WGS for predicting resistance against first- and second-line drugs, as well as providing a phylogenetic characterization of TB drug-resistant isolates circulating in Mexico.
全基因组测序（WGS）已被提议作为一种用于诊断结核病（TB）耐药性的工具；然而，在耐药率显著较高的国家，关于其有效性的研究很少。因此，本研究旨在评估 WGS 识别墨西哥流行地区结核分枝杆菌分离株中与耐药相关突变的有效性。结果表明，在分析的 35 株耐多药分离株中，表型药敏试验和多态性之间的一致性值分别为异烟肼 94%、利福平 97%、乙胺丁醇 90%和吡嗪酰胺 82%。还可以识别出 8 株潜在的广泛耐药（XDR）和 1 株 XDR。29 株分离株被分类为 L4 型，并且鉴定出两个传播群。结果表明，WGS 具有预测一线和二线药物耐药性的潜在用途，并提供了在墨西哥流行的耐结核药物分离株的系统发育特征。
