Wiester M J, Tepper J S, King M E, Ménache M G, Costa D L
Toxicology Branch, Inhalation Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.
Toxicol Appl Pharmacol. 1988 Oct;96(1):140-6. doi: 10.1016/0041-008x(88)90256-6.
Ozone uptake was assessed in awake, spontaneously breathing Fischer-344 Sprague-Dawley, and Long-Evans rats and Hartley guinea pigs to provide data on the dosimetry of O3 in small laboratory animals. This information is needed for extrapolation of O3 toxicity data from experimental animals to man. Breathing measurements and O3 exposure data were obtained using a head-out body plethysmograph connected to a nose-only exposure system. The fractional uptake of O3 was computed by measuring flow and O3 concentration both upstream and downstream from the nose. The quantity of O3 removed by the animal, O2 consumption, CO2 production, and tidal breathing measurements were automatically recorded once each minute. All animal types were exposed for 1 hr to 0.3 ppm O3. Other Fischer-344 rats were also exposed for 1 hr to 0.0 or to 0.6 ppm O3. Exposure concentrations of O3 had no significant effect on percentage O3 uptake in Fischer-344 rats. Results showed that percentage O3 uptake (47%) did not differ significantly among the three strains of rats nor between the rats and the guinea pigs. Similarly, percentage O3 uptake was independent of animal age, lung weight, and lung volume as well as normal variations encountered in the resting breathing measures. However, species-specific ventilation and O3 concentration were the primary determinants of the accumulated lung dose of O3 during the exposures.
在清醒、自主呼吸的Fischer-344大鼠、斯普拉格-道利大鼠、长-伊文斯大鼠和哈特利豚鼠中评估臭氧摄取情况，以获取小型实验动物中臭氧剂量测定的数据。从实验动物外推臭氧毒性数据到人类需要这些信息。使用连接到仅鼻暴露系统的头部外身体体积描记器获取呼吸测量值和臭氧暴露数据。通过测量鼻子上游和下游的流量和臭氧浓度来计算臭氧的摄取分数。动物去除的臭氧量、氧气消耗、二氧化碳产生和潮气量测量值每分钟自动记录一次。所有动物类型均暴露于0.3 ppm臭氧1小时。其他Fischer-344大鼠也暴露于0.0或0.6 ppm臭氧1小时。臭氧暴露浓度对Fischer-344大鼠的臭氧摄取百分比没有显著影响。结果表明，三种大鼠品系之间以及大鼠和豚鼠之间的臭氧摄取百分比（47%）没有显著差异。同样，臭氧摄取百分比与动物年龄、肺重量、肺体积以及静息呼吸测量中遇到的正常变化无关。然而，特定物种的通气和臭氧浓度是暴露期间肺部累积臭氧剂量的主要决定因素。
