静息与运动状态下人体吸入臭氧的剂量及效应生物标志物：与静息大鼠的比较
Biomarkers of Dose and Effect of Inhaled Ozone in Resting versus Exercising Human Subjects: Comparison with Resting Rats.
作者信息
Hatch Gary E, McKee John, Brown James, McDonnell William, Seal Elston, Soukup Joleen, Slade Ralph, Crissman Kay, Devlin Robert
机构信息
National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina.
出版信息
Biomark Insights. 2013 May 19;8:53-67. doi: 10.4137/BMI.S11102. Print 2013.
To determine the influence of exercise on pulmonary dose of inhaled pollutants, we compared biomarkers of inhaled ozone (O3) dose and toxic effect between exercise levels in humans, and between humans and rats. Resting human subjects were exposed to labeled O3 ((18)O3, 0.4 ppm, for 2 hours) and alveolar O3 dose measured as the concentration of excess (18)O in cells and extracellular material of nasal, bronchial, and bronchoalveolar lavage fluid (BALF). We related O3 dose to effects (changes in BALF protein, LDH, IL-6, and antioxidant substances) measurable in the BALF. A parallel study of resting subjects examined lung function (FEV1) changes following O3. Subjects exposed while resting had (18)O concentrations in BALF cells that were 1/5th of those of exercising subjects and directly proportional to the amount of O3 breathed during exposure. Quantitative measures of alveolar O3 dose and toxicity that were observed previously in exercising subjects were greatly reduced or non-observable in O3 exposed resting subjects. Resting rats and resting humans were found to have a similar alveolar O3 dose.
为了确定运动对吸入污染物肺部剂量的影响，我们比较了人类不同运动水平之间以及人类与大鼠之间吸入臭氧（O₃）剂量和毒性作用的生物标志物。静息状态的人类受试者暴露于标记的O₃（¹⁸O₃，0.4 ppm，持续2小时），肺泡O₃剂量通过鼻腔、支气管和支气管肺泡灌洗液（BALF）细胞及细胞外物质中过量¹⁸O的浓度来测量。我们将O₃剂量与BALF中可测量的效应（BALF蛋白、乳酸脱氢酶、白细胞介素-6和抗氧化物质的变化）相关联。一项针对静息受试者的平行研究检测了O₃暴露后肺功能（第一秒用力呼气容积，FEV₁）的变化。静息状态下暴露的受试者BALF细胞中的¹⁸O浓度是运动受试者的1/5，且与暴露期间吸入的O₃量成正比。先前在运动受试者中观察到的肺泡O₃剂量和毒性的定量指标在O₃暴露的静息受试者中大幅降低或未观察到。发现静息大鼠和静息人类的肺泡O₃剂量相似。
Zotero 插件