Baily G G, Mason P R, Trijssenar F E, Lyons N F
Department of Medicine, University of Zimbabwe, School of Medicine, Avondale, Harare.
Trans R Soc Trop Med Hyg. 1988;82(2):295-9. doi: 10.1016/0035-9203(88)90451-8.
An ELISA system was developed to assist with the diagnosis of neurocysticercosis. Antigens were prepared from the fluid, the protoscolex and the cyst wall of cysticerci dissected from infected pigs. The fluid antigen was tested both fresh and after freeze-drying and one year's storage. Sera from patients with proven neurocysticercosis were most reactive using the fluid and least reactive using the cyst wall antigens. Freeze-drying and prolonged storage of fluid antigen did not reduce its reactivity and repeated testing of sera with this antigen gave optical density values within 15% of the original value. Using this ELISA system positive results were obtained from all patients with active neurocysticercosis. Sera from patients with calcified cysts were usually non-reactive or marginally reactive to the fluid antigen.
开发了一种酶联免疫吸附测定（ELISA）系统以辅助神经囊尾蚴病的诊断。抗原取自从感染猪体内解剖出的囊尾蚴的囊液、原头节和囊壁。对新鲜的、冻干并储存一年后的囊液抗原进行了检测。经证实患有神经囊尾蚴病的患者血清与囊液抗原反应最强，与囊壁抗原反应最弱。囊液抗原的冻干和长期储存并未降低其反应性，用该抗原对血清进行重复检测得到的光密度值在原始值的15%以内。使用该ELISA系统，所有活动性神经囊尾蚴病患者均获得阳性结果。钙化囊肿患者的血清通常对囊液抗原无反应或仅有微弱反应。
