Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM) EA 4312, Normandie Université - Université de Rouen, Évreux, 27000, Evreux, France.
Laboratoire de Biotechnologie et Chimie Marines (LBCM) EA 3884, IUEM, Université de Bretagne-Sud, 56100, Lorient, France.
Sci Rep. 2019 Dec 27;9(1):20203. doi: 10.1038/s41598-019-56666-7.
Microbial endocrinology has demonstrated for more than two decades, that eukaryotic substances (hormones, neurotransmitters, molecules of the immune system) can modulate the physiological behavior of bacteria. Among them, the hormones/neurotransmitters, epinephrine (Epi) and norepinephrine (NE), released in case of stress, physical effort or used in medical treatment, were shown to be able to modify biofilm formation in various bacterial species. In the present study, we have evaluated the effect of Epi on motility, adhesion, biofilm formation and virulence of Pseudomonas aeruginosa, a bacterium linked to many hospital-acquired infections, and responsible for chronic infection in immunocompromised patients including persons suffering from cystic fibrosis. The results showed that Epi increased adhesion and biofilm formation of P. aeruginosa, as well as its virulence towards the Galleria mellonella larvae in vivo model. Deciphering the sensor of this molecule in P. aeruginosa and the molecular mechanisms involved may help to find new strategies of treatment to fight against this bacterium.
微生物内分泌学已经证实了二十多年，真核物质（激素、神经递质、免疫系统分子）可以调节细菌的生理行为。其中，在应激、体力活动或用于治疗的情况下释放的激素/神经递质肾上腺素（Epi）和去甲肾上腺素（NE）被证明能够改变各种细菌物种的生物膜形成。在本研究中，我们评估了 Epi 对铜绿假单胞菌（一种与许多医院获得性感染相关的细菌，也是免疫功能低下患者慢性感染的原因，包括囊性纤维化患者）的运动性、黏附性、生物膜形成和毒力的影响。结果表明，Epi 增加了铜绿假单胞菌的黏附和生物膜形成，以及其对活体模型中美洲大蠊幼虫的毒力。解析 P. aeruginosa 中这种分子的传感器和涉及的分子机制可能有助于寻找新的治疗策略来对抗这种细菌。
