Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Laboratory of Aquaculture and Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Ghent University, Gent, Belgium.
J Fish Dis. 2019 Apr;42(4):477-487. doi: 10.1111/jfd.12934. Epub 2019 Jan 29.
In this study, we evaluated the impact of the catecholamines on growth, swimming motility, biofilm formation and some virulence factors activities of pathogenic Yersinia ruckeri. Norepinephrine and dopamine (at 100 µM) significantly increased the growth of Y. ruckeri in culture media containing serum. An increase in swimming motility of the pathogen was found following the exposure to the hormones; however, no effect was seen on caseinase, phospholipase and haemolysin productions. Further, antagonists for the catecholamine receptors were observed to block some of the influences of the catecholamines. Indeed, the effects of catecholamines were inhibited by chlorpromazine (the dopaminergic receptor antagonist) for dopamine, labetalol (α-and β-adrenergic receptor antagonist) and phenoxybenzamine (the α-adrenergic receptor antagonist) for norepinephrine, but propranolol (the β-adrenergic receptor antagonist) showed no effect. Pretreatment of Y. ruckeri with the catecholamines resulted in a significant enhancement of its virulence towards rainbow trout and the antagonists could neutralize the effect of the stress hormones in vivo. In summary, our results show that the catecholamines increase the virulence of Y. ruckeri which is pathogenic to trout through increasing the motility, biofilm formation and growth.
在这项研究中，我们评估了儿茶酚胺对致病性鲁氏耶尔森氏菌生长、游动能力、生物膜形成和一些毒力因子活性的影响。去甲肾上腺素和多巴胺（100μM）显著增加了含血清培养基中 Y. 鲁氏耶尔森氏菌的生长。在接触激素后，病原体的游动能力增加；然而，对酪蛋白酶、磷脂酶和溶血素的产生没有影响。此外，儿茶酚胺受体的拮抗剂被观察到阻断儿茶酚胺的一些影响。事实上，儿茶酚胺的作用被氯丙嗪（多巴胺能受体拮抗剂）抑制，用于多巴胺；拉贝洛尔（α 和 β 肾上腺素能受体拮抗剂）和酚妥拉明（α 肾上腺素能受体拮抗剂）用于去甲肾上腺素，但普萘洛尔（β 肾上腺素能受体拮抗剂）没有效果。用儿茶酚胺预处理 Y. 鲁氏耶尔森氏菌显著增强了其对彩虹鳟鱼的毒力，拮抗剂可以中和体内应激激素的作用。总之，我们的结果表明，儿茶酚胺通过增加运动性、生物膜形成和生长来增加 Y. 鲁氏耶尔森氏菌的毒力，这种菌对鳟鱼具有致病性。
