6-脱氧-6-精氨酸修饰壳聚糖的制备、表征及抗菌性能。
Preparation, characterization and antibacterial properties of 6-deoxy-6-arginine modified chitosan.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Pilot National Laboratory for Marine Science and Technology, Qingdao 266235, China.
出版信息
Carbohydr Polym. 2020 Feb 15;230:115635. doi: 10.1016/j.carbpol.2019.115635. Epub 2019 Nov 21.
In this study, 6-deoxy-6-arginine modified chitosan (DAC), was synthesized and characterized by Fourier Transform Infrared Spectroscopy (FTIR), H and C nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and elemental analysis. The arginine was grafted onto C6 groups of chitosan. Antibacterial activity of DAC against gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated at concentration between 0.02 mg/mL and 10 mg/mL. Cell viability assessment was estimated in vitro with Caco-2 and L929 cells. Water solubility of DAC at different pH was also evaluated. The results showed that the minimum inhibitory concentration (MICs) of DAC against S. aureus and E. coli were 0.078 mg/mL and 0.312 mg/mL, respectively. The minimum bactericidal concentration (MBC) against S. aureus and E. coli was 0.625 mg/mL. The cytotoxicity of chitosan and DAC was not significantly different. It demonstrated that DAC might be a potential safe antibacterial agent.
在这项研究中，合成并通过傅里叶变换红外光谱（FTIR）、氢核和碳核磁共振（NMR）、差示扫描量热法（DSC）和元素分析对 6-脱氧-6-精氨酸修饰壳聚糖（DAC）进行了表征。精氨酸被接枝到壳聚糖的 C6 基团上。在 0.02 mg/mL 至 10 mg/mL 的浓度下，研究了 DAC 对革兰氏阴性菌大肠杆菌（E. coli）和革兰氏阳性菌金黄色葡萄球菌（S. aureus）的抗菌活性。通过 Caco-2 和 L929 细胞体外评估了细胞活力评估。还评估了 DAC 在不同 pH 值下的水溶性。结果表明，DAC 对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度（MICs）分别为 0.078 mg/mL 和 0.312 mg/mL。对金黄色葡萄球菌和大肠杆菌的最小杀菌浓度（MBC）分别为 0.625 mg/mL。壳聚糖和 DAC 的细胞毒性没有显著差异。这表明 DAC 可能是一种有潜力的安全抗菌剂。
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