N-羧乙基壳聚糖在生物医学中的潜在应用：制备、表征、生物学特性。
Potential use of N-carboxyethylchitosan in biomedical applications: Preparation, characterization, biological properties.
机构信息
Textile Research Division, National Research Centre, 33 El Bohouthst. (Former El Tahrir St.), Dokki, P.O.12622, Giza, Egypt.
Chemistry Department, Faculty of Girls for Arts, Science and Education, Ain Shams University, Heliopolis 11757, Cairo, Egypt.
出版信息
Int J Biol Macromol. 2020 Apr 15;149:664-671. doi: 10.1016/j.ijbiomac.2020.01.299. Epub 2020 Jan 31.
N-carboxyethylchitosan (CECS) was successfully prepared via Michael addition reaction of chitosan (CS) with acrylic acid in water. The structure of CECS was characterized by Fourier transform Infra-Red spectrometry (FT-IR), HNMR, elemental analysis, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC). Antibacterial activity of CECS was evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by using minimum inhibition concentration (MIC). The results showed that the prepared CECS soluble in water at wide range of pH values. In addition, it has amorphous character improve its chemical reactivity than CS itself, in addition it has been showed stronger antibacterial activity than chitosan itself due to the presence of both -COOH and -NH groups and the CECS shows higher antibacterial activity towards S. aureus than E. coli. Finally, the cytotoxicity of CECS has been evaluated through Cell viability assay, which confirm that CECS is non-toxic and tissue compatible like CS.
N-羧乙基壳聚糖（CECS）通过壳聚糖（CS）与丙烯酸在水中的迈克尔加成反应成功制备。通过傅里叶变换红外光谱（FT-IR）、HNMR、元素分析、X 射线衍射（XRD）、热重分析（TGA）和差示扫描量热法（DSC）对 CECS 的结构进行了表征。通过最小抑菌浓度（MIC）评价了 CECS 对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抑菌活性。结果表明，所制备的 CECS 在很宽的 pH 值范围内可溶于水。此外，它具有无定形特征，比 CS 本身提高了其化学反应性，此外，由于存在 -COOH 和 -NH 基团，它表现出比壳聚糖本身更强的抗菌活性，CECS 对金黄色葡萄球菌的抗菌活性高于大肠杆菌。最后，通过细胞活力测定法评价了 CECS 的细胞毒性，证实 CECS 像 CS 一样无毒且组织相容性好。
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