Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, Qingdao, 266003, China.
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, Qingdao, 266003, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
Carbohydr Polym. 2020 Feb 15;230:115698. doi: 10.1016/j.carbpol.2019.115698. Epub 2019 Nov 30.
Two fucosylated chondroitin sulfates FCS and FCS were isolated from the sea cucumber Holothuria polii and Holothuria tubulosa, respectively. The NMR spectroscopy and HILIC-FTMS methods were applied for their detailed structural characterization. Chemical analysis indicated that the two FCSs all contained a chondroitin sulfate backbone chondroitin sulfate-like core and fucosyl branches of α-L-Fuc2,4S, α-L-Fuc4S or α-L-Fuc3,4S linked to O-3 of glucuronic acid residues. The main branches of FCS and FCS were monofucose, and the small amounts of di-, tri- and tetrafucose with α-1,3-linkage type were also detected. Finally, we investigated the immunomodulatory function of FCS and FCS in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that FCS and FCS had beneficial effects on hematopoietic function recovery in CTX-induced bone marrow suppression mice. Notably, the α-L-Fuc2,4S was more important to the activity than α-L-Fuc3,4S. These results provided basis for developing the drugs to reduce side effects of chemotherapy.
两种岩藻糖基硫酸软骨素 FCS 和 FCS 分别从海参 Holothuria polii 和 Holothuria tubulosa 中分离得到。采用 NMR 光谱和 HILIC-FTMS 方法对其进行了详细的结构表征。化学分析表明，两种 FCS 均含有硫酸软骨素主链、类似硫酸软骨素的核心以及α-L-Fuc2,4S、α-L-Fuc4S 或α-L-Fuc3,4S 连接到葡萄糖醛酸残基 O-3 的岩藻糖支链。FCS 和 FCS 的主要支链为单岩藻糖，也检测到少量以α-1,3 键型连接的二、三、四岩藻糖。最后，我们研究了 FCS 和 FCS 在环磷酰胺（CTX）诱导的免疫抑制小鼠模型中的免疫调节功能。结果表明，FCS 和 FCS 对 CTX 诱导的骨髓抑制小鼠的造血功能恢复有有益作用。值得注意的是，α-L-Fuc2,4S 比α-L-Fuc3,4S 对活性更重要。这些结果为开发减少化疗副作用的药物提供了依据。
