G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok 690022, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok 690022, Russia.
Carbohydr Polym. 2020 Feb 15;230:115629. doi: 10.1016/j.carbpol.2019.115629. Epub 2019 Nov 16.
The research described here focused on the effect of sulfated red algal polysaccharides (κ-, κ/β-, ι/κ-carrageenan) individually and in combination with lipopolysaccharide (LPS) on the synthesis of prostaglandin E (PGE) and cytokines (interleukin [IL]-1β and IL-6) in whole blood model in vitro. The results demonstrated that, at high concentrations, carrageenans have substantial ability to modulate PGE synthesis and stimulate IL-1β and IL-6 synthesis. A low degree of sulfate and high molecular weight were a prerequisite for the ability of carrageenans to modulate PGE synthesis. Further, we investigated the ability of the carrageenans alone and in combination with casein to affect bile salt permeability through an artificial membrane imitating the gastrointestinal barrier. The least sulfated κ/β-carrageenan could retain bile salt permeation the most but less efficiently than cholestyramine. The polysaccharides did not affect pancreatic lipase activity. Our data confirm a possible mechanism of the cholesterol-reducing properties of carrageenan.
本研究重点探讨了硫酸化红藻多糖（κ-、κ/β-、ι/κ-卡拉胶）单独及与脂多糖（LPS）组合对体外全血模型中环前列腺素 E（PGE）和细胞因子（白细胞介素 [IL]-1β 和 IL-6）合成的影响。结果表明，在高浓度下，卡拉胶具有显著调节 PGE 合成和刺激 IL-1β 和 IL-6 合成的能力。低硫酸化程度和高分子量是卡拉胶调节 PGE 合成能力的前提。此外，我们还研究了卡拉胶单独及与酪蛋白组合通过模拟胃肠道屏障的人工膜对胆汁盐通透性的影响。硫酸化程度最低的 κ/β-卡拉胶保留胆汁盐渗透的能力最强，但不如考来烯胺有效。多糖对胰脂肪酶活性没有影响。我们的数据证实了卡拉胶降低胆固醇特性的可能机制。
