G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, 690022 Vladivostok, Russia.
N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Lavrentjev Ave. 9, 630090 Novosibirsk, Russia.
Mar Drugs. 2020 Sep 4;18(9):458. doi: 10.3390/md18090458.
The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses. For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared. The ability of PEC to scavenge NO depended on the content of the κ-CGN in the PEC. The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition. The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice. The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
评估了κ-卡拉胶（κ-CGN）和壳聚糖（CH）的各种组成的聚电解质复合物（PEC）与初始多糖在可比剂量下的免疫调节活性。为此，制备了两种可溶性 PEC 形式，一种是 CH 过量（CH：CGN 质量比为 10：1），另一种是 CGN 过量（CH：CGN 质量比为 1：10）。PEC 清除 NO 的能力取决于 PEC 中 κ-CGN 的含量。通过初始 κ-CGN 的活性，无论其组成如何，确定 PEC 诱导外周血单个核细胞合成促炎（肿瘤坏死因子-α（TNF-α））和抗炎（白细胞介素-10（IL-10））细胞因子的能力。使用组胺、刀豆蛋白 A 和绵羊红细胞免疫诱导的小鼠炎症试验研究了 PEC 和初始化合物的抗炎活性。在组胺诱导的渗出性炎症中观察到 PEC 以及初始多糖 κ-CGN 和 CH 的最高活性，这与吞噬细胞（即巨噬细胞和中性粒细胞）的激活直接相关。
