School of Environmental & Rural Science, University of New England, Armidale, 2351, Australia.
School of Science & Technology, University of New England, Armidale, 2351, Australia.
Carbohydr Polym. 2020 Feb 15;230:115645. doi: 10.1016/j.carbpol.2019.115645. Epub 2019 Nov 19.
Xylan hydrolysis into xylo-oligosaccharides (XOS) was evaluated both in the gizzard and ileum of broiler chickens, and by a 2-step in vitro digestion assay that simulated the pH, temperature and time period of the gastric and small intestine (SI) phases. Twelve dietary treatments with varying soluble and insoluble xylan levels, either with or without supplemental xylanase, were fed to broiler chickens (n = 576) for the in situ analysis, and were exposed to the in vitro assay. Relatedness of the two methods was strong for determination of XOS production in all dietary treatments for X, X, X, X and X respectively, in both the gastric (r = 0.980, 0.853, 0.894, 0.870 and 0.951) and small intestine phase (r = 0.957, 0.923, 0.940, 0.970, 0.969) (P < 0.05). Consequently, the in vitro assay was used to illustrated the diversity of XOS production across different batches of wheat and barley in the presence of xylanase.
木聚糖水解为木二糖（XOS）在肉鸡的肌胃和回肠中进行了评估，并通过两步体外消化试验模拟了胃和小肠（SI）阶段的 pH、温度和时间。在体内分析中，用含有或不含有补充木聚糖酶的不同可溶性和不溶性木聚糖水平的 12 种饲粮处理方法喂养肉鸡（n=576），并暴露于体外分析中。对于所有饲粮处理方法，在胃（r=0.980、0.853、0.894、0.870 和 0.951）和小肠阶段（r=0.957、0.923、0.940、0.970、0.969）（P<0.05）中，两种方法对于 XOS 生产的相关性均很强。因此，体外试验用于说明在有木聚糖酶存在的情况下，不同批次的小麦和大麦中 XOS 生产的多样性。
