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Cryo 电子断层扫描和小角 X 射线散射研究多态性保护 Dps-DNA 共晶

Polymorphic Protective Dps-DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering.

机构信息

National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.

出版信息

Biomolecules. 2019 Dec 26;10(1):39. doi: 10.3390/biom10010039.

DOI:10.3390/biom10010039
PMID:31888079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7023142/
Abstract

Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps-DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps-DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods.

摘要

细胞内特定组蛋白样 Dps 蛋白的合成迅速增加，该蛋白能与 DNA 结合以保护基因组免受有害因素的影响，从而导致细胞内结晶——这是目前生命科学领域最奇特的过程之一。然而，二十多年来，Dps-DNA 共晶的实际结构细节仍不确定。低温电子断层扫描和小角 X 射线散射显示，共晶随缓冲参数的变化而发生多态性修饰。体外形成了两种不同类型的 Dps-DNA 共晶：三斜晶系和立方晶系。三维重构揭示了立方共晶中的 DNA 和 Dps 分子，两种方法都一致地确定了立方晶格的晶胞参数。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/0e7907cc055b/biomolecules-10-00039-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/7df6e7949696/biomolecules-10-00039-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/61a1495d4fea/biomolecules-10-00039-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/66444a442bd7/biomolecules-10-00039-g0A3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/f2b658487d5a/biomolecules-10-00039-g0A4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/efde27f89093/biomolecules-10-00039-g0A5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/4a87fe15032b/biomolecules-10-00039-g0A6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/6b8f544eb482/biomolecules-10-00039-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/ea04e3882868/biomolecules-10-00039-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/f14b00fa0450/biomolecules-10-00039-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/0e7907cc055b/biomolecules-10-00039-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/7df6e7949696/biomolecules-10-00039-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/61a1495d4fea/biomolecules-10-00039-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/66444a442bd7/biomolecules-10-00039-g0A3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/f2b658487d5a/biomolecules-10-00039-g0A4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/efde27f89093/biomolecules-10-00039-g0A5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/4a87fe15032b/biomolecules-10-00039-g0A6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/6b8f544eb482/biomolecules-10-00039-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/ea04e3882868/biomolecules-10-00039-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/f14b00fa0450/biomolecules-10-00039-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7465/7023142/0e7907cc055b/biomolecules-10-00039-g004.jpg

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