使用同步辐射进行多晶体数据收集，以 Dps 的低对称晶体为例。
Multi-crystal data collection using synchrotron radiation as exemplified with low-symmetry crystals of Dps.
机构信息
Structure of Matter, Semenov FRC for Chemical Physics, RAS, 4 Kosygina Street, Moscow 119991, Russian Federation.
European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France.
出版信息
Acta Crystallogr F Struct Biol Commun. 2020 Nov 1;76(Pt 11):568-576. doi: 10.1107/S2053230X20012571. Epub 2020 Oct 8.
Abstract
Multi-crystal data collection using synchrotron radiation was successfully applied to determine the three-dimensional structure of a triclinic crystal form of Dps from Escherichia coli at 2.0 Å resolution. The final data set was obtained by combining 261 partial diffraction data sets measured from crystals with an average size of approximately 5 µm. The most important features of diffraction data measurement and processing for low-symmetry crystals are discussed.
摘要
利用同步辐射进行多晶体数据收集，成功地确定了来自大肠杆菌的 Dps 的三斜晶型的三维结构，分辨率为 2.0 Å。最终数据集是通过组合来自平均大小约为 5 µm 的晶体的 261 个部分衍射数据集获得的。讨论了低对称晶体的衍射数据测量和处理的最重要特征。
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