De Jesus Rener, Dedeles Gina
The Graduate School, University of Santo Tomas, España, Manila 1008, Philippines.
Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila 1008, Philippines.
Data Brief. 2019 Dec 5;28:104951. doi: 10.1016/j.dib.2019.104951. eCollection 2020 Feb.
The microtiter plate biofilm formation assay is a method for the study of early biofilm formation on abiotic surfaces. It is a colorimetric technique that uses dyes, such as crystal violet, to stain attached biofilms and to quantify by using an absorbance microtiter plate reader. In this data, we evaluated the ability of 12 isolated from soil and milk powder samples for their production of biofilms after a total of 48 hr incubation period in the 96-well microtiter plate. The biofilm production was induced by initially exposing them in diluted tryptic soy broth at its first 24 hr and then replacing with freshly prepared double strength broth for the next incubation period at 30 °C. The optical densities of the bacterial growth in the wells were read at the absorbance wavelength of 630 nm while the stained biofilms that solubilized in absolute ethanol were read at 570 nm. The biofilm measurements were calculated and the degree of biofilm production of each isolate was classified according to biofilm formation categories adapted from previous researchers. Therefore, the assay concluded the negative impact of group by ability to form biofilms on abiotic surfaces, such as food contact surfaces in food production industries and the wide application of the current methods in research and industrial fields.
微量滴定板生物膜形成测定法是一种用于研究非生物表面早期生物膜形成的方法。它是一种比色技术，使用诸如结晶紫等染料对附着的生物膜进行染色，并通过使用吸光微量滴定板读数器进行定量。在本数据中，我们评估了从土壤和奶粉样品中分离出的12株菌株在96孔微量滴定板中总共孵育48小时后形成生物膜的能力。生物膜的产生是通过在最初的24小时将它们暴露于稀释的胰蛋白胨大豆肉汤中，然后在接下来的孵育期于30°C用新制备的双倍强度肉汤替换来诱导的。在630nm的吸光波长下读取孔中细菌生长的光密度，而在570nm下读取溶解在无水乙醇中的染色生物膜的光密度。计算生物膜测量值，并根据先前研究人员采用的生物膜形成类别对每个分离株的生物膜产生程度进行分类。因此，该测定得出结论，该组通过在非生物表面（如食品生产行业中的食品接触表面）形成生物膜的能力以及当前方法在研究和工业领域的广泛应用产生负面影响。
