Soedarsono Soedarsono, Dolli Parluhutan
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Clin Tuberc Other Mycobact Dis. 2019 Dec 4;18:100138. doi: 10.1016/j.jctube.2019.100138. eCollection 2020 Feb.
Tuberculosis (TB) is caused by infection with . Sometimes it is difficult to distinguish between active and inactive pulmonary TB based on chest X-ray images. TB biomarkers are needed to diagnose active TB when TB bacilli are not detected either through microscopic examination, culture or geneXpert MTB/Rif. Neopterin can be used as an additional diagnosis and therapy monitoring as well as assessing activation of macrophage cells. This study aims to examine and comparing serum neopterin levels in new cases of pulmonary TB with positive AFB and inactive pulmonary TB.
This was an analytic study with Cross-Sectional design conducted in Dr. Soetomo General Hospital Surabaya, Indonesia. The subjects of the study were the new cases of pulmonary TB with positive AFB and inactive pulmonary TB who met the inclusion and exclusion criteria. Blood serum was collected and examined to measure neopterin serum levels in patients by ELISA technique.
There was a significant difference of mean neopterin serum levels between the new cases of pulmonary TB with positive AFB and inactive pulmonary TB with a p-value <0.001 (74.92 nmol/l vs. 9.84 nmol/l). There was a significant difference in serum neopterin levels in each AFB grading in the new cases of pulmonary TB with positive AFB with a p-value <0.001 (60.10 nmol/l in AFB 1+, 80.81 nmol/l in AFB 2+, and 110.70 nmol/l in AFB 3+).
Serum neopterin levels in the new cases of pulmonary TB with positive AFB were higher than inactive pulmonary TB.
结核病（TB）由感染……引起。有时，根据胸部X光图像很难区分活动性和非活动性肺结核。当通过显微镜检查、培养或GeneXpert MTB/Rif未检测到结核杆菌时，需要结核生物标志物来诊断活动性肺结核。蝶呤可用于辅助诊断、治疗监测以及评估巨噬细胞的激活情况。本研究旨在检测并比较痰涂片抗酸杆菌（AFB）阳性的肺结核新发病例与非活动性肺结核患者的血清蝶呤水平。
这是一项在印度尼西亚泗水苏托莫博士综合医院进行的采用横断面设计的分析性研究。研究对象为符合纳入和排除标准的痰涂片抗酸杆菌阳性的肺结核新发病例与非活动性肺结核患者。采集血清样本，采用酶联免疫吸附测定（ELISA）技术检测患者血清中的蝶呤水平。
痰涂片抗酸杆菌阳性的肺结核新发病例与非活动性肺结核患者的血清蝶呤平均水平存在显著差异，p值<0.001（74.92纳摩尔/升 vs. 9.84纳摩尔/升）。痰涂片抗酸杆菌阳性的肺结核新发病例中，各AFB分级的血清蝶呤水平存在显著差异，p值<0.001（AFB 1+为60.10纳摩尔/升，AFB 2+为80.81纳摩尔/升，AFB 3+为1,10.70纳摩尔/升）。
痰涂片抗酸杆菌阳性的肺结核新发病例的血清蝶呤水平高于非活动性肺结核患者。
