Pathological Diagnostic Center, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang 157011, PR China.
Graduate School of Mudanjiang Medical University, Mudanjiang 157011, PR China.
Life Sci. 2020 Feb 1;242:117240. doi: 10.1016/j.lfs.2019.117240. Epub 2019 Dec 28.
Lycium barbarum polysaccharides (LBP) are derived from Wolfberry and have antioxidant activities. This study aimed to evaluate the efficacy of LBP for kidney injury in a rat model of sepsis. Male rats were divided randomly to control group (Con), LPS group (LPS), ulinastatin group (ULI), low dose LBP group (LBP-1), middle dose LBP group (LBP-2) and high dose LBP group (LBP-3). After intraperitoneal injection of LPS (5 mg/kg) to make sepsis model (LPS group), 10,000 U/kg ulinastatin were given in ULI group, and 200, 400 and 800 mg/kg LBP was given in LBP-1, -2, -3 group, respectively. Serum IL-1β, IL-6, IL-8, TNF-α and NF-κB levels were measured by ELISA. Nrf2, Keap1, NF-κB, HO-1 and NQO1 expression levels were detected by PCR and Western blot analysis. We found that LBP decreased the levels of NF-κB and pro-inflammatory cytokines while attenuated kidney injury. In addition, LBP regulated Keap1-Nrf2/ARE signaling pathway in the kidney. In conclusion, LBP attenuates inflammation injury in the kidney via possible regulation of Keap1-Nrf2/ARE signaling.
枸杞多糖（LBP）来源于枸杞，具有抗氧化活性。本研究旨在评估 LBP 对脓毒症大鼠模型肾损伤的疗效。雄性大鼠随机分为对照组（Con）、LPS 组（LPS）、乌司他丁组（ULI）、低剂量 LBP 组（LBP-1）、中剂量 LBP 组（LBP-2）和高剂量 LBP 组（LBP-3）。经腹腔注射 LPS（5mg/kg）建立脓毒症模型（LPS 组）后，ULI 组给予 10000U/kg 乌司他丁，LBP-1、-2、-3 组分别给予 200、400 和 800mg/kg LBP。采用 ELISA 法检测血清中 IL-1β、IL-6、IL-8、TNF-α和 NF-κB 水平，PCR 和 Western blot 分析检测 Nrf2、Keap1、NF-κB、HO-1 和 NQO1 表达水平。结果发现，LBP 降低了 NF-κB 和促炎细胞因子的水平，同时减轻了肾损伤。此外，LBP 调节了肾脏中的 Keap1-Nrf2/ARE 信号通路。综上所述，LBP 通过可能调节 Keap1-Nrf2/ARE 信号通路减轻肾脏炎症损伤。
