Khoshkhounejad Mehrfam, Sobhi Afshar Mahsa, Jabalameli Fereshteh, Emaneini Mohammad, Sharifian Mohammadreza
Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Eur J Dent. 2019 Oct;13(4):514-520. doi: 10.1055/s-0039-3401369. Epub 2019 Dec 31.
This study aimed to evaluate the cytotoxicity of minimum antibacterial values of medicaments used in endodontic regeneration on stem cells.
"Minimum inhibitory concentration," "minimum bactericidal concentration," and "minimum biofilm inhibitory concentration" of triple and double antibiotic paste, a modified triple antibiotic paste (minocycline replaced by clindamycin), Augmentin, and calcium hydroxide were determined using (ATCC 29212) by microtiter plate method. Direct cytotoxic effects of drugs were evaluated by lactate dehydrogenase and water-soluble tetrazolium salt-1 assays using stem cells of apical papilla obtained from immature third molars via enzymatic digestion.
Data were analyzed using IBM SPSS Statistics 24, one-way analysis of variance and post hoc comparisons. The statistical power was set at < 0.05.
All medicaments caused similar cytotoxicity and cell proliferation at "minimum inhibitory concentration" ( > 0.05) except Augmentin which was significantly more toxic than others ( < 0.05). At "minimum bactericidal concentration," calcium hydroxide was more toxic than other drugs ( < 0.001), but its adverse effect on cell proliferation was the same as Augmentin ( > 0.05). Triple and double antibiotic paste revealed similar favorable effects in terms of toxicity and proliferation rate at most of the tested concentrations ( > 0.05). At "minimum biofilm inhibitory concentration" both the modified paste and Augmentin caused less proliferation rate than triple and double antibiotic paste ( < 0.001and < 0.05, respectively) and Augmentin induced more cytotoxicity ( < 0.05).
Considering the antimicrobial potency, triple antibiotic paste seems to be the safest drug for the stem cells of apical papilla, while Augmentin may have some adverse effects.
本研究旨在评估牙髓再生中使用的药物最低抗菌值对干细胞的细胞毒性。
采用微量滴定板法，使用（美国典型培养物保藏中心29212）测定三联和双联抗生素糊剂、改良三联抗生素糊剂（用克林霉素替代米诺环素）、阿莫西林克拉维酸钾和氢氧化钙的“最低抑菌浓度”“最低杀菌浓度”和“最低生物膜抑制浓度”。通过乳酸脱氢酶和水溶性四氮唑盐-1试验，使用经酶消化从未成熟第三磨牙获得的根尖乳头干细胞评估药物的直接细胞毒性作用。
使用IBM SPSS Statistics 24进行数据分析，采用单因素方差分析和事后比较。设定统计学显著性水平为<0.05。
除阿莫西林克拉维酸钾毒性显著高于其他药物（<0.05）外，所有药物在“最低抑菌浓度”时均引起相似的细胞毒性和细胞增殖（>0.05）。在“最低杀菌浓度”时，氢氧化钙比其他药物毒性更大（<0.001），但其对细胞增殖的不良影响与阿莫西林克拉维酸钾相同（>0.05）。三联和双联抗生素糊剂在大多数测试浓度下在毒性和增殖率方面显示出相似的良好效果（>0.05）。在“最低生物膜抑制浓度”时，改良糊剂和阿莫西林克拉维酸钾的增殖率均低于三联和双联抗生素糊剂（分别为<0.001和<0.05），且阿莫西林克拉维酸钾诱导的细胞毒性更大（<0.05）。
考虑到抗菌效力，三联抗生素糊剂似乎是对根尖乳头干细胞最安全的药物，而阿莫西林克拉维酸钾可能有一些不良影响。
