Labban Nawaf, Yassen Ghaeth H, Windsor L Jack, Platt Jeffrey A
Department of Prosthetic Dental Science, King Saud University, Riyadh, Saudi Arabia; Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA.
Dent Traumatol. 2014 Dec;30(6):429-34. doi: 10.1111/edt.12108. Epub 2014 Mar 19.
The purpose of this in vitro study was to evaluate the effects of intracanal medicaments commonly used in endodontic regeneration on the survival of human dental pulp cells (DPCs).
DPCs were cultured and exposed to either no medicament treatment or low concentrations (0.3-5 mg ml(-1) ) of calcium hydroxide [Ca(OH)2 ], triple antibiotic paste (TAP), or double antibiotic paste (DAP) for 3 days. After that, toxicity to the DPCs was determined by lactate dehydrogenase activity assays (LDH) and cell proliferation was measured by colorimetric assays (WST-1). Two-way anova followed by Fisher's protected least significant differences was used for statistical analyses (α = 0.05).
The group-by-concentration interactions were significant for the LDH and WST-1 assays (P < 0.0001). For the LDH assays, only the highest tested concentration (5 mg ml(-1) ) of Ca(OH)2 and TAP caused significant toxicity to the DPCs compared with the untreated control, while four tested concentrations of DAP (0.5, 1, 2.5, and 5 mg ml(-1) ) caused significant toxicity to the DPCs compared with the untreated control. For the WST-1 assays, the highest concentrations that did not negatively affect the proliferation rate of DAP, TAP and Ca(OH)2 were 0.3, 2, and 2.5 mg ml(-1) , respectively.
The low concentrations of intracanal medicaments tested in this study were not cytotoxic in cultured cells. However, these concentrations are much lower than the concentrations that have been advocated in endodontic regeneration. Furthermore, the negative effects of TAP on DPCs were detected at lower concentrations by using the WST-1 assays than by measuring the LDH release.
本体外研究旨在评估牙髓再生中常用的根管内药物对人牙髓细胞（DPCs）存活的影响。
培养DPCs，并将其暴露于无药物处理或低浓度（0.3 - 5 mg ml⁻¹）的氢氧化钙[Ca(OH)₂]、三联抗生素糊剂（TAP）或双联抗生素糊剂（DAP）中3天。之后，通过乳酸脱氢酶活性测定（LDH）确定对DPCs的毒性，并通过比色测定法（WST - 1）测量细胞增殖。采用双向方差分析及Fisher保护最小显著差异法进行统计分析（α = 0.05）。
对于LDH和WST - 1测定，组间浓度交互作用具有显著性（P < 0.0001）。对于LDH测定，与未处理的对照组相比，仅测试的最高浓度（5 mg ml⁻¹）的Ca(OH)₂和TAP对DPCs产生显著毒性，而四种测试浓度的DAP（0.5、1、2.5和5 mg ml⁻¹）与未处理的对照组相比对DPCs产生显著毒性。对于WST - 1测定，对DAP、TAP和Ca(OH)₂增殖率无负面影响的最高浓度分别为0.3、2和2.5 mg ml⁻¹。
本研究中测试的低浓度根管内药物在培养细胞中无细胞毒性。然而，这些浓度远低于牙髓再生中所提倡的浓度。此外，通过WST - 1测定法比通过测量LDH释放检测到TAP对DPCs的负面影响时的浓度更低。
