Pandey Aparna, Bhushan Jagat, Joshi Rajesh Kumar, Uppal Amandeep Singh, Angrup Archana, Kansal Shubhangi
Department of Conservative Dentistry and Endodontics, Rayat Bahra Dental College and Hospital, Mohali, Punjab, India.
Department of Conservative Dentistry and Endodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, India.
J Conserv Dent Endod. 2024 Jul;27(7):750-754. doi: 10.4103/JCDE.JCDE_219_24. Epub 2024 Jul 8.
The aim of the study was to assess and evaluate the antimicrobial effectiveness of chitosan nanoparticles (CSNPs) with calcium hydroxide in the elimination of .
Using the broth microdilution method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of calcium hydroxide and CSNPs were measured. The antibiofilm effect of calcium hydroxide and CSNPs against biofilm was qualitatively analyzed using a crystal violet assay. A 7-day-old biofilms of grown on dentine discs were assigned to the following three groups ( = 11 dentine discs), normal saline (group I), calcium hydroxide (group II), and CSNPs (group III). Quantification of live and dead cells using confocal microscopy was done to evaluate the antibiofilm efficacy of the medicaments included in the study.
MIC of calcium hydroxide and CSNPs against was observed at 2.5 mg/mL and 0.31 mg/mL, respectively. MBC of calcium hydroxide and CSNPs was observed at 2.5 mg/mL and 0.31 mg/mL, respectively. Using Crystal Violet (CV) assay, calcium hydroxide and CSNPs showed biofilm inhibition at concentrations of 2.5 mg/mL and 0.625 mg/mL, respectively. Confocal laser scanning microscopy analysis found that both calcium hydroxide and CSNPs showed a significant decrease in viable cells at their MBC values compared to the control group's normal saline. CSNPs showed a significantly lower percentage of live cells than calcium hydroxide ( < 0.05).
The study results reveal that the antimicrobial efficacy of CSNPs is better than calcium hydroxide and normal saline against biofilm.
本研究旨在评估和评价壳聚糖纳米颗粒（CSNPs）与氢氧化钙联合使用在消除……方面的抗菌效果。
采用肉汤微量稀释法，测定氢氧化钙和CSNPs的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。使用结晶紫测定法对氢氧化钙和CSNPs对……生物膜的抗生物膜作用进行定性分析。将在牙本质盘上生长7天的……生物膜分为以下三组（每组 = 11个牙本质盘），生理盐水（I组）、氢氧化钙（II组）和CSNPs（III组）。使用共聚焦显微镜对活细胞和死细胞进行定量分析，以评估本研究中所包含药物的抗生物膜功效。
观察到氢氧化钙和CSNPs对……的MIC分别为2.5 mg/mL和0.31 mg/mL。观察到氢氧化钙和CSNPs的MBC分别为2.5 mg/mL和0.31 mg/mL。使用结晶紫（CV）测定法，氢氧化钙和CSNPs分别在浓度为2.5 mg/mL和0.625 mg/mL时显示出生物膜抑制作用。共聚焦激光扫描显微镜分析发现，与对照组的生理盐水相比，氢氧化钙和CSNPs在其MBC值时均显示活细胞显著减少。CSNPs显示活细胞百分比显著低于氢氧化钙（P < 0.05）。
研究结果表明，CSNPs对……生物膜的抗菌效果优于氢氧化钙和生理盐水。
