Castells Matías, Bertoni Estefany, Caffarena Rubén Darío, Casaux María Laura, Schild Carlos, Victoria Matías, Riet-Correa Franklin, Giannitti Federico, Parreño Viviana, Colina Rodney
Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.
Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud Animal, Ruta 50 km 11, La Estanzuela 64988, Colonia, Uruguay.
Viruses. 2019 Dec 27;12(1):32. doi: 10.3390/v12010032.
Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the genus were identified, including , (3 samples each) and an unclassified species (19 samples). The unclassified species was characterized as a novel species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation.
影响牛的病毒感染给全球畜牧业带来了经济损失，但对于美洲肠道牛星状病毒（BoAstV）的传播、致病性和遗传多样性却知之甚少。这项工作的目的是描述乌拉圭奶牛中肠道BoAstV的流行情况和遗传多样性。2015年7月至2017年5月期间，共收集了457份奶牛犊粪便和43份肠道内容物，通过逆转录聚合酶链反应（RT-PCR）进行检测，随后对聚合酶和衣壳区域进行测序和系统发育分析。26%（128/500）的样本呈阳性。在该属中鉴定出三个不同的种，包括[具体种名1]、[具体种名2]（各3个样本）和一个未分类的种（19个样本）。该未分类的种被鉴定为一个新的种。BoAstV在乌拉圭奶牛中传播，具有高度的遗传多样性。肠道BoAstV感染牛的最终临床病理意义需要进一步研究。
