Yan Kai, Li Chao-Chuang, Wang Yu, Wang Xiao-Quan, Wang Zhi-Min, Wei Da-Yong, Tang Qing-Lin
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.
Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China.
Plant Biotechnol (Tokyo). 2018 Dec 25;35(4):357-363. doi: 10.5511/plantbiotechnology.18.0824a.
is an important vegetable and condiment crop widely grown in Asia, and the yield and quality of its product organs are affected by flowering time. AGAMOUS-LIKE18-1 (AGL18-1) belongs to a member of MADS-domain transcription factors, which play vital roles in flowering time control, but the biological role of in () has not been thoroughly revealed in flowering regulatory network. In this study, expressed highly in inflorescence and flower, but slightly in root, stem and leaf. The sense and anti-sense transgenic lines of were generated and showed that functioned as a flowering inhibitor and depressed growth of lateral branching. During the vegetative phase, induced another flowering repressor () but inhibited the flowering signal integrator of () in . Whereas, during the flower developmental phase, both and () were down-regulated by . By contrast, was promoted by , while () was independent of . Additionally, () was highly induced by . These results will provide valuable information for clarifying the molecular mechanism of in mediating flowering time.
是一种在亚洲广泛种植的重要蔬菜和调味品作物,其产品器官的产量和质量受开花时间的影响。AGAMOUS-LIKE18-1(AGL18-1)属于MADS结构域转录因子成员,在开花时间控制中起重要作用,但在开花调控网络中,其在()中的生物学作用尚未完全揭示。在本研究中,在花序和花中高表达,但在根、茎和叶中表达较低。构建了的正义和反义转基因株系,结果表明其作为开花抑制因子发挥作用,并抑制侧枝生长。在营养生长阶段,诱导另一个开花抑制因子(),但在中抑制开花信号整合因子()。然而,在花发育阶段,和()均被下调。相比之下,被促进,而()与之无关。此外,()被高度诱导。这些结果将为阐明在介导开花时间中的分子机制提供有价值的信息。