and Translocation in B-cell Non-Hodgkin Lymphoma Cases.

, and genes are the most commonly oncogenes involved in B-Cell lymphomas. Translocations of these oncogenes are associated with an aggressive clinical course. This study aims to elucidate the patterns of , and gene aberrations among Malaysian B-cell Non-Hodgkin Lymphoma (NHL) using fluorescence hybridization (FISH). Eighty-one B-cell NHL tissue blocks were retrieved between the year 2011 to 2015 and investigated using immunohistochemistry and interphase FISH dual colour break-apart probes of , , and . A significant difference was detected between the nodal and extranodal sites in all the (p=0.01), (p=0.03) and (p=0.006) cases except for (p=0.2). Our study showed that had the highest gene translocation while had the most mixed aberrations of gain copies and translocation, however no mixed aberrations of gain copies and translocation was found in . None of the mixed gain copies and translocation was found in any of the germinal centre B-cell (GCB) subtype of Diffuse Large B-cell Lymphoma, however, five were found in and gene in the non-GCB subtype; while mixed gain copies and translocation cases of gene was found in the Follicular Lymphoma cases only. The study found interesting findings of , and gene aberrations between nodal and extranodal sites. This information might benefit future study in predicting prognosis and determine effective therapeutic strategies in the multi-ethnic populations of Malaysia as well as the Asian population.