Salam Dayang Sharyati Datu Abdul, Thit Ei Ei, Teoh Siew Hoon, Tan Soo Yong, Peh Suat Cheng, Cheah Shiau-Chuen
Faculty of Medicine and Health Sciences, UCSI University, Kuala Lumpur, Malaysia.
Advanced Molecular Pathology Laboratory, SingHealth Tissue Repository, Singapore.
J Cancer. 2020 Jan 1;11(1):190-198. doi: 10.7150/jca.36954. eCollection 2020.
, and genes are the most commonly oncogenes involved in B-Cell lymphomas. Translocations of these oncogenes are associated with an aggressive clinical course. This study aims to elucidate the patterns of , and gene aberrations among Malaysian B-cell Non-Hodgkin Lymphoma (NHL) using fluorescence hybridization (FISH). Eighty-one B-cell NHL tissue blocks were retrieved between the year 2011 to 2015 and investigated using immunohistochemistry and interphase FISH dual colour break-apart probes of , , and . A significant difference was detected between the nodal and extranodal sites in all the (p=0.01), (p=0.03) and (p=0.006) cases except for (p=0.2). Our study showed that had the highest gene translocation while had the most mixed aberrations of gain copies and translocation, however no mixed aberrations of gain copies and translocation was found in . None of the mixed gain copies and translocation was found in any of the germinal centre B-cell (GCB) subtype of Diffuse Large B-cell Lymphoma, however, five were found in and gene in the non-GCB subtype; while mixed gain copies and translocation cases of gene was found in the Follicular Lymphoma cases only. The study found interesting findings of , and gene aberrations between nodal and extranodal sites. This information might benefit future study in predicting prognosis and determine effective therapeutic strategies in the multi-ethnic populations of Malaysia as well as the Asian population.
、 和 基因是B细胞淋巴瘤中最常见的致癌基因。这些致癌基因的易位与侵袭性临床病程相关。本研究旨在利用荧光原位杂交（FISH）阐明马来西亚B细胞非霍奇金淋巴瘤（NHL）中 、 和 基因畸变模式。2011年至2015年间检索了81个B细胞NHL组织块，并使用免疫组织化学以及 、 、 和 的间期FISH双色断裂分离探针进行研究。除 （p = 0.2）外，在所有 （p = 0.01）、 （p = 0.03）和 （p = 0.006）病例中，淋巴结和结外部位之间均检测到显著差异。我们的研究表明， 具有最高的基因易位，而 具有最多的增益拷贝和易位混合畸变，然而在 中未发现增益拷贝和易位的混合畸变。在弥漫性大B细胞淋巴瘤的生发中心B细胞（GCB）亚型中均未发现增益拷贝和易位的混合情况，然而，在非GCB亚型的 和 基因中发现了5例；而 基因的增益拷贝和易位混合病例仅在滤泡性淋巴瘤病例中发现。该研究发现了淋巴结和结外部位之间 、 和 基因畸变的有趣结果。这些信息可能有助于未来在马来西亚多民族人群以及亚洲人群中预测预后和确定有效治疗策略的研究。
